BMRB Entry 28045

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28045

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Title:
A short motif in the N-terminal region of a-synuclein plays a critical role in aggregation and function
Deposition date:
2019-11-20
Original release date:
2020-04-03
Authors:
Doherty, Ciaran; Ulamec, Sabine; Maya Martinez, Roberto Carlos; Good, Sarah; Makepeace, Jemma; Khan, Nasir; van Oosten-Hawle, Patricija; Radford, Sheena; Brockwell, David
Citation:

Citation: Doherty, Ciaran; Ulamec, Sabine; Maya Martinez, Roberto Carlos; Good, Sarah; Makepeace, Jemma; Khan, Nasir; van Oosten-Hawle, Patricija; Radford, Sheena; Brockwell, David. "A short motif in the N-terminal region of alpha-synuclein is critical for both aggregation and function"  Nat. Struct. Mol. Biol. 27, 249-259 (2020).
PubMed: 32157247

Assembly members:

Assembly members:
GS-P1P2_human_alpha-synuclein, polymer, 140 residues, 13948.23 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GS-P1P2_human_alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKESGSGS GSKTGSGSGSGSGSGSGKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts368
15N chemical shifts121
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human alpha-Synuclein (P1P2-GS)1

Entities:

Entity 1, human alpha-Synuclein (P1P2-GS) 140 residues - 13948.23 Da.

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUSERGLYSERGLYSER
5   GLYSERLYSTHRGLYSERGLYSERGLYSER
6   GLYSERGLYSERGLYSERGLYLYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: P1P2-GS human alpha-synuclein, [U-100% 13C; U-100% 15N], 250 ± 5 uM

Assignment_conditions: ionic strength: 0.028 M; pH: 4.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicAssignment_conditions
3D HNCOsample_1isotropicAssignment_conditions
3D HNCACBsample_1isotropicAssignment_conditions
3D HN(CO)CACBsample_1isotropicAssignment_conditions
3D HN(CA)NNHsample_1isotropicAssignment_conditions
3D HN(COCA)NNHsample_1isotropicAssignment_conditions
3D HNNH-TOCSYsample_1isotropicAssignment_conditions
3D HN(CA)COsample_1isotropicAssignment_conditions

Software:

Analysis-ccpNMR vv.2.4.2, CCPN - data analysis

nmrPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v3.5, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Ascend Aeon 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks