BMRB Entry 28040

Name: Backbone and CB Chemical Shift Assignments of the Methanocaldococcus jannaschii Spt5-KOW domain
Published: 2023-01-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28040

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and CB Chemical Shift Assignments of the Methanocaldococcus jannaschii Spt5-KOW domain

Deposition date:

2019-11-11

Original release date:

2023-01-09

Authors:

Zuber, Philipp; Schweimer, Kristian; Knauer, Stefan

Citation:

Citation: Zuber, Philipp; Daviter, Tina; Heissmann, Ramona; Persau, Ulrike; Schweimer, Kristian; Knauer, Stefan. "Structural and thermodynamic analyses of the beta-to-alpha transformation in RfaH reveal principles of fold-switching proteins" Elife 11, e76630-e76630 (2022).
PubMed: 36255050

Assembly members:

Assembly members:
MjSpt5-KOW, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: 2190 Superkingdom: Archaea Kingdom: not available Genus/species: Methanocaldococcus jannaschii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETGb1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MjSpt5-KOW: GAMGKKIIENIEKGDVVEII AGPFKGERAKVIRVDKHKEE VTLELENAAVPIPITLPVEG VKIVSKHKD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	189
15N chemical shifts	62
1H chemical shifts	62

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MjSpt5-KOW	1

Entities:

Entity 1, MjSpt5-KOW 69 residues - Formula weight is not available

Residues 1-4 result from an engineered TEV-protease cleavage site; residues 5-69 correspond to residues 83-147 of MjSpt5

1

GLY

ALA

MET

GLY

LYS

ILE

GLU

ASN

2

ILE

GLU

LYS

GLY

ASP

VAL

GLU

ILE

3

ALA

GLY

PRO

PHE

LYS

GLY

GLU

ARG

ALA

LYS

4

VAL

ILE

ARG

VAL

ASP

LYS

HIS

LYS

GLU

5

VAL

THR

LEU

GLU

LEU

GLU

ASN

ALA

VAL

6

PRO

ILE

PRO

ILE

THR

LEU

PRO

VAL

GLU

GLY

7

VAL

LYS

ILE

VAL

SER

LYS

HIS

LYS

ASP

Samples:

sample_1: MjSpt5-KOW, [U-99% 13C; U-99% 15N], 1 mM; potassium phosphate 20 mM; sodium chloride 100 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.139 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C ctHSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN vv3.5 pl5, Bruker Biospin - collection

NMRViewJ vv9.2.0-b2, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Ascend Aeon 900 MHz

UNP	Q57818
AlphaFold	Q57818