BMRB Entry 28035
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR28035
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Citation: Shi, Xiangyan; Prasanna, Chinmayi; Pervushin, Konstantin; Nordenskiold, Lars. "Solid-state NMR 13C, 15N assignments of human histone H3 in the nucleosome core particle" Biomol. NMR Assignments 14, 99-104 (2020).
Assembly members:
human_histone_H3, polymer, 135 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3a
Entity Sequences (FASTA):
human_histone_H3: ARTKQTARKSTGGKAPRKQL
ATKAARKSAPATGGVKKPHR
YRPGTVALREIRRYQKSTEL
LIRKLPFQRLVREIAQDFKT
DLRFQSSAVMALQEACEAYL
VGLFEDTNLCAIHAKRVTIM
PKDIQLARRIRGERA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 387 |
| 15N chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human histone H3 | 1 |
Entities:
Entity 1, human histone H3 135 residues - Formula weight is not available
| 1 | ALA | ARG | THR | LYS | GLN | THR | ALA | ARG | LYS | SER | ||||
| 2 | THR | GLY | GLY | LYS | ALA | PRO | ARG | LYS | GLN | LEU | ||||
| 3 | ALA | THR | LYS | ALA | ALA | ARG | LYS | SER | ALA | PRO | ||||
| 4 | ALA | THR | GLY | GLY | VAL | LYS | LYS | PRO | HIS | ARG | ||||
| 5 | TYR | ARG | PRO | GLY | THR | VAL | ALA | LEU | ARG | GLU | ||||
| 6 | ILE | ARG | ARG | TYR | GLN | LYS | SER | THR | GLU | LEU | ||||
| 7 | LEU | ILE | ARG | LYS | LEU | PRO | PHE | GLN | ARG | LEU | ||||
| 8 | VAL | ARG | GLU | ILE | ALA | GLN | ASP | PHE | LYS | THR | ||||
| 9 | ASP | LEU | ARG | PHE | GLN | SER | SER | ALA | VAL | MET | ||||
| 10 | ALA | LEU | GLN | GLU | ALA | CYS | GLU | ALA | TYR | LEU | ||||
| 11 | VAL | GLY | LEU | PHE | GLU | ASP | THR | ASN | LEU | CYS | ||||
| 12 | ALA | ILE | HIS | ALA | LYS | ARG | VAL | THR | ILE | MET | ||||
| 13 | PRO | LYS | ASP | ILE | GLN | LEU | ALA | ARG | ARG | ILE | ||||
| 14 | ARG | GLY | GLU | ARG | ALA |
Samples:
sample_1: human histone H3, [U-100% 13C; U-100% 15N], 3.1 mg; histone H4 2.2 mg; human histone H2A 2.7 mg; human histone H2B 2.7 mg; Widom 601 DNA 8.6 mg; H2O 35.9 mg
sample_conditions_1: ionic strength: 20 mM; pH: 7.5; pressure: 1 atm; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D CC DARR with 20 ms mixing time | sample_1 | isotropic | sample_conditions_1 |
| 2D CC DARR with 100 ms mixing time | sample_1 | isotropic | sample_conditions_1 |
| 2D NCA | sample_1 | isotropic | sample_conditions_1 |
| 2D NCO | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX with 75 ms CC DARR | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX with 75 ms CC DARR | sample_1 | isotropic | sample_conditions_1 |
| 3D CANCO | sample_1 | isotropic | sample_conditions_1 |
| 2D CC DREAM | sample_1 | isotropic | sample_conditions_1 |
| 2D NcaCX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX with 20 ms CC DARR | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX with 20 ms CC DARR | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.116, Goddard - data analysis
TOPSPIN v3.5pl6, Bruker Biospin - collection
NMRPipe v3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz