BMRB Entry 28033

Name: Backbone assignments for the Asciz QT2-4/LC8 complex
Published: 2020-03-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28033

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments for the Asciz QT2-4/LC8 complex

Deposition date:

2019-10-24

Original release date:

2020-03-05

Authors:

Reardon, Patrick; Barbar, Elisar; Jara, Kayla; Smith, Delaney

Citation:

Citation: Reardon, Patrick; Jara, Kayla; Rolland, Amber; Smith, Delaney; Hoang, Hanh; Prell, James; Barbar, Elisar. "The dynein light chain 8 (LC8) binds predominantly "in-register" to a multivalent intrinsically disordered partner" J. Biol. Chem. 295, 4912-4922 (2020).
PubMed: 32139510

Assembly members:

Assembly members:
QT2-4, polymer, 76 residues, Formula weight is not available
LC8, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET2Zt2-1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
QT2-4: GAMHMNEVLAPLLRDIETQT PDTRGDIGTMTDDFPEEQEP VAVGSHFHAYSETEPMFDLQ TSAHMYTQTCDDLFEE
LC8: GAHMSDRKAVIKNADMSEEM QQDAVDCATQALEKYNIEKD IAAYIKKEFDKKYNPTWHCI VGRNFGSYVTHETRHFIYFY LGQVAILLFKSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	149
15N chemical shifts	53
1H chemical shifts	53

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	QT2-4, chain 1	1
2	QT2-4, chain 2	1
3	LC8, chain 1-1	2
4	LC8, chain 1-2	2
5	LC8, chain 2-1	2
6	LC8, chain 2-2	2
7	LC8, chain 3-1	2
8	LC8, chain 4-2	2

Entities:

Entity 1, QT2-4, chain 1 76 residues - Formula weight is not available

1

GLY

ALA

MET

HIS

MET

ASN

GLU

VAL

LEU

ALA

2

PRO

LEU

ARG

ASP

ILE

GLU

THR

GLN

THR

3

PRO

ASP

THR

ARG

GLY

ASP

ILE

GLY

THR

MET

4

THR

ASP

PHE

PRO

GLU

GLN

GLU

PRO

5

VAL

ALA

VAL

GLY

SER

HIS

PHE

HIS

ALA

TYR

6

SER

GLU

THR

GLU

PRO

MET

PHE

ASP

LEU

GLN

7

THR

SER

ALA

HIS

MET

TYR

THR

GLN

THR

CYS

8

ASP

LEU

PHE

GLU

Entity 2, LC8, chain 1-1 92 residues - Formula weight is not available

1

GLY

ALA

HIS

MET

SER

ASP

ARG

LYS

ALA

VAL

2

ILE

LYS

ASN

ALA

ASP

MET

SER

GLU

MET

3

GLN

ASP

ALA

VAL

ASP

CYS

ALA

THR

GLN

4

ALA

LEU

GLU

LYS

TYR

ASN

ILE

GLU

LYS

ASP

5

ILE

ALA

TYR

ILE

LYS

GLU

PHE

ASP

6

LYS

TYR

ASN

PRO

THR

TRP

HIS

CYS

ILE

7

VAL

GLY

ARG

ASN

PHE

GLY

SER

TYR

VAL

THR

8

HIS

GLU

THR

ARG

HIS

PHE

ILE

TYR

PHE

TYR

9

LEU

GLY

GLN

VAL

ALA

ILE

LEU

PHE

LYS

10

SER

GLY

Samples:

sample_1: QT2-4, [U-13C; U-15N; U-2H], 0.5 mM; LC8 2.0 mM; beta-mercaptoethanol 10 mM; sodium phosphate 10 mM; sodium chloride 10 mM; sodium azide 1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks