BMRB Entry 28026

Name: TNPO1_328-381
Published: 2020-04-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28026

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

TNPO1_328-381

Deposition date:

2019-10-04

Original release date:

2020-04-03

Authors:

bourgeois, benjamin; madl, tobias

Citation:

Citation: Bourgeois, Benjamin; Hutten, Saskia; Gottschalk, Benjamin; Hofweber, Mario; Richter, Gesa; Sternat, Julia; Abou-Ajram, Claudia; Gobl, Christoph; Leitinger, Gerd; Graier, Wolfgang; Dormann, Dorothee; Madl, Tobias. "Nonclassical nuclear localization signals mediate nuclear import of CIRBP" Proc. Natl. Acad. Sci. U.S.A. 117, 8503-8514 (2020).
PubMed: 32234784

Assembly members:

Assembly members:
TNPO1, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TNPO1: GAMGDVEEDETIPDSEQDIR PRFHRSRTVAQQHDEDGIEE EDDDDDEIDDDDTISDW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	46
1H chemical shifts	45

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TNPO1_328-381	1

Entities:

Entity 1, TNPO1_328-381 57 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

ASP

VAL

GLU

ASP

GLU

2

THR

ILE

PRO

ASP

SER

GLU

GLN

ASP

ILE

ARG

3

PRO

ARG

PHE

HIS

ARG

SER

ARG

THR

VAL

ALA

4

GLN

HIS

ASP

GLU

ASP

GLY

ILE

GLU

5

GLU

ASP

GLU

ILE

ASP

6

ASP

THR

ILE

SER

ASP

TRP

Samples:

sample_1: TNPO1, [U-99% 13C; U-99% 15N], 500 uM; D2O 10%; TRIS 50 mM; sodium chloride 150 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CCPN, Bruker Biospin, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection, peak picking, processing

NMR spectrometers:

GE Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks