BMRB Entry 28016

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28016
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Title:
NMR resonance assignments of the hazelnut allergen isoform Cor a 1.0404
Deposition date:
2019-09-13
Original release date:
2019-11-08
Authors:
Fuehrer, Sebastian; Zeindl, Ricarda; Tollinger, Martin
Citation:

Citation: Fuehrer, Sebastian; Zeindl, Ricarda; Tollinger, Martin. "NMR resonance assignments of the four isoforms of the hazelnut allergen Cor a 1.04"  Biomol. NMR Assignments 14, 45-49 (2020).
PubMed: 31691092

Assembly members:

Assembly members:
Cor_a_1.0404, polymer, 160 residues, 17443.85 Da.

Natural source:

Natural source:   Common Name: European Hazlenut   Taxonomy ID: 13451   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Corylus avellana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b (+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cor_a_1.0404: GVFSYEDEATSVIPPARLFK SFVLDADNLIPKVAPQHFTS AENLEGNGGPGTIKKITFAE GNEFKYMKHKVEEIDHANFK YCYSIIEGGPLGHTLEKIPY EIKMAAAPHGGGSILKITSK YHTKGNASINEEEIKAGKEK AAGLFKAVEAYLLAHPDAYC

Data sets:
Data typeCount
13C chemical shifts636
15N chemical shifts151
1H chemical shifts955

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cor a 1.04041

Entities:

Entity 1, Cor a 1.0404 160 residues - 17443.85 Da.

1   GLYVALPHESERTYRGLUASPGLUALATHR
2   SERVALILEPROPROALAARGLEUPHELYS
3   SERPHEVALLEUASPALAASPASNLEUILE
4   PROLYSVALALAPROGLNHISPHETHRSER
5   ALAGLUASNLEUGLUGLYASNGLYGLYPRO
6   GLYTHRILELYSLYSILETHRPHEALAGLU
7   GLYASNGLUPHELYSTYRMETLYSHISLYS
8   VALGLUGLUILEASPHISALAASNPHELYS
9   TYRCYSTYRSERILEILEGLUGLYGLYPRO
10   LEUGLYHISTHRLEUGLULYSILEPROTYR
11   GLUILELYSMETALAALAALAPROHISGLY
12   GLYGLYSERILELEULYSILETHRSERLYS
13   TYRHISTHRLYSGLYASNALASERILEASN
14   GLUGLUGLUILELYSALAGLYLYSGLULYS
15   ALAALAGLYLEUPHELYSALAVALGLUALA
16   TYRLEULEUALAHISPROASPALATYRCYS

Samples:

sample_1: Cor a 1.0404, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM; DTT 2 mM

sample_2: Cor a 1.0404, [U-99% 15N], 0.5 mM; sodium phosphate 20 mM; DTT 2 mM

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQC NH2 onlysample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Agilent DD2 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks