BMRB Entry 28009

Name: HRASG12VGMPPNP
Published: 2021-08-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28009

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HRASG12VGMPPNP

Deposition date:

2019-09-05

Original release date:

2021-08-17

Authors:

He, Yanan; Chen, Yingwei; Ruan, Biao; Simmerman, Richard; Chen, Yihong; Wang, Ruixue; Garagusi, Kyle; Godoy-Ruiz, Raquel; King, Harlan; Custer, Gregory; Gallagher, Travis; Rozak, David; Solomon, Melani; Muro, Silvia; Weber, David; Fuerst, Thomas; Toth, Eric A; Bryan, Philp; Orban, John

Citation:

Citation: Chen, Yingwei; Toth, Eric; Ruan, Biao; Choi, Eun Jung; Simmerman, Richard; Chen, Yihong; He, Yanan; Wang, Ruixue; Godoy-Ruiz, Raquel; King, Harlan; Custer, Gregory; Travis Gallagher, D.; Rozak, David; Solomon, Melani; Muro, Silvia; Weber, David; Orban, John; Fuerst, Thomas; Bryan, Philip. "Engineering subtilisin proteases that specifically degrade active RAS" Commun. Biol. 4, 299-299 (2021).
PubMed: 33674772

Assembly members:

Assembly members:
HRASG12VGMPPNP, polymer, 170 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pG58

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HRASG12VGMPPNP: GPHMTEYKLVVVGAVGVGKS ALTIQLIQNHFVDEYDPTIE DSYRKQVVIDGETCLLDILD TAGQEEYSAMRDQYMRTGEG FLCVFAINNTKSFEDIHQYR EQIKRVKDSDDVPMVLVGNK CDLAARTVESRQAQDLARSY GIPYIETSAKTRQGVEDAFY TLVREIRQHK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	400
15N chemical shifts	126
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HRASG12VGMPPNP	1

Entities:

Entity 1, HRASG12VGMPPNP 170 residues - Formula weight is not available

1	GLY	PRO	HIS	MET	THR	GLU	TYR	LYS	LEU	VAL
2	VAL	VAL	GLY	ALA	VAL	GLY	VAL	GLY	LYS	SER
3	ALA	LEU	THR	ILE	GLN	LEU	ILE	GLN	ASN	HIS
4	PHE	VAL	ASP	GLU	TYR	ASP	PRO	THR	ILE	GLU
5	ASP	SER	TYR	ARG	LYS	GLN	VAL	VAL	ILE	ASP
6	GLY	GLU	THR	CYS	LEU	LEU	ASP	ILE	LEU	ASP
7	THR	ALA	GLY	GLN	GLU	GLU	TYR	SER	ALA	MET
8	ARG	ASP	GLN	TYR	MET	ARG	THR	GLY	GLU	GLY
9	PHE	LEU	CYS	VAL	PHE	ALA	ILE	ASN	ASN	THR
10	LYS	SER	PHE	GLU	ASP	ILE	HIS	GLN	TYR	ARG
11	GLU	GLN	ILE	LYS	ARG	VAL	LYS	ASP	SER	ASP
12	ASP	VAL	PRO	MET	VAL	LEU	VAL	GLY	ASN	LYS
13	CYS	ASP	LEU	ALA	ALA	ARG	THR	VAL	GLU	SER
14	ARG	GLN	ALA	GLN	ASP	LEU	ALA	ARG	SER	TYR
15	GLY	ILE	PRO	TYR	ILE	GLU	THR	SER	ALA	LYS
16	THR	ARG	GLN	GLY	VAL	GLU	ASP	ALA	PHE	TYR
17	THR	LEU	VAL	ARG	GLU	ILE	ARG	GLN	HIS	LYS

Samples:

sample_1: HRASG12VGMPPNP, [U-100% 13C; U-100% 15N], 0.5 mM; HEPES 20 mM; NaCl 50 mM; MgCl2 5 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	GLY	PRO	HIS	MET	THR	GLU	TYR	LYS	LEU	VAL
2	VAL	VAL	GLY	ALA	VAL	GLY	VAL	GLY	LYS	SER
3	ALA	LEU	THR	ILE	GLN	LEU	ILE	GLN	ASN	HIS
4	PHE	VAL	ASP	GLU	TYR	ASP	PRO	THR	ILE	GLU
5	ASP	SER	TYR	ARG	LYS	GLN	VAL	VAL	ILE	ASP
6	GLY	GLU	THR	CYS	LEU	LEU	ASP	ILE	LEU	ASP
7	THR	ALA	GLY	GLN	GLU	GLU	TYR	SER	ALA	MET
8	ARG	ASP	GLN	TYR	MET	ARG	THR	GLY	GLU	GLY
9	PHE	LEU	CYS	VAL	PHE	ALA	ILE	ASN	ASN	THR
10	LYS	SER	PHE	GLU	ASP	ILE	HIS	GLN	TYR	ARG
11	GLU	GLN	ILE	LYS	ARG	VAL	LYS	ASP	SER	ASP
12	ASP	VAL	PRO	MET	VAL	LEU	VAL	GLY	ASN	LYS
13	CYS	ASP	LEU	ALA	ALA	ARG	THR	VAL	GLU	SER
14	ARG	GLN	ALA	GLN	ASP	LEU	ALA	ARG	SER	TYR
15	GLY	ILE	PRO	TYR	ILE	GLU	THR	SER	ALA	LYS
16	THR	ARG	GLN	GLY	VAL	GLU	ASP	ALA	PHE	TYR
17	THR	LEU	VAL	ARG	GLU	ILE	ARG	GLN	HIS	LYS

1	GLY	PRO	HIS	MET	THR	GLU	TYR	LYS	LEU	VAL
2	VAL	VAL	GLY	ALA	VAL	GLY	VAL	GLY	LYS	SER
3	ALA	LEU	THR	ILE	GLN	LEU	ILE	GLN	ASN	HIS
4	PHE	VAL	ASP	GLU	TYR	ASP	PRO	THR	ILE	GLU
5	ASP	SER	TYR	ARG	LYS	GLN	VAL	VAL	ILE	ASP
6	GLY	GLU	THR	CYS	LEU	LEU	ASP	ILE	LEU	ASP
7	THR	ALA	GLY	GLN	GLU	GLU	TYR	SER	ALA	MET
8	ARG	ASP	GLN	TYR	MET	ARG	THR	GLY	GLU	GLY
9	PHE	LEU	CYS	VAL	PHE	ALA	ILE	ASN	ASN	THR
10	LYS	SER	PHE	GLU	ASP	ILE	HIS	GLN	TYR	ARG
11	GLU	GLN	ILE	LYS	ARG	VAL	LYS	ASP	SER	ASP
12	ASP	VAL	PRO	MET	VAL	LEU	VAL	GLY	ASN	LYS
13	CYS	ASP	LEU	ALA	ALA	ARG	THR	VAL	GLU	SER
14	ARG	GLN	ALA	GLN	ASP	LEU	ALA	ARG	SER	TYR
15	GLY	ILE	PRO	TYR	ILE	GLU	THR	SER	ALA	LYS
16	THR	ARG	GLN	GLY	VAL	GLU	ASP	ALA	PHE	TYR
17	THR	LEU	VAL	ARG	GLU	ILE	ARG	GLN	HIS	LYS

1	GLY	PRO	HIS	MET	THR	GLU	TYR	LYS	LEU	VAL
2	VAL	VAL	GLY	ALA	VAL	GLY	VAL	GLY	LYS	SER
3	ALA	LEU	THR	ILE	GLN	LEU	ILE	GLN	ASN	HIS
4	PHE	VAL	ASP	GLU	TYR	ASP	PRO	THR	ILE	GLU
5	ASP	SER	TYR	ARG	LYS	GLN	VAL	VAL	ILE	ASP
6	GLY	GLU	THR	CYS	LEU	LEU	ASP	ILE	LEU	ASP
7	THR	ALA	GLY	GLN	GLU	GLU	TYR	SER	ALA	MET
8	ARG	ASP	GLN	TYR	MET	ARG	THR	GLY	GLU	GLY
9	PHE	LEU	CYS	VAL	PHE	ALA	ILE	ASN	ASN	THR
10	LYS	SER	PHE	GLU	ASP	ILE	HIS	GLN	TYR	ARG
11	GLU	GLN	ILE	LYS	ARG	VAL	LYS	ASP	SER	ASP
12	ASP	VAL	PRO	MET	VAL	LEU	VAL	GLY	ASN	LYS
13	CYS	ASP	LEU	ALA	ALA	ARG	THR	VAL	GLU	SER
14	ARG	GLN	ALA	GLN	ASP	LEU	ALA	ARG	SER	TYR
15	GLY	ILE	PRO	TYR	ILE	GLU	THR	SER	ALA	LYS
16	THR	ARG	GLN	GLY	VAL	GLU	ASP	ALA	PHE	TYR
17	THR	LEU	VAL	ARG	GLU	ILE	ARG	GLN	HIS	LYS