BMRB Entry 27999

Name: Structural basis for client recognition and activity of Hsp40 chaperones
Published: 2019-11-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27999

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis for client recognition and activity of Hsp40 chaperones

Deposition date:

2019-08-16

Original release date:

2019-11-07

Authors:

Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos

Citation:

Citation: Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos. "Structural basis for client recognition and activity of Hsp40 chaperones" Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
Sis1_SBD, polymer, 158 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sis1_SBD: EEETVQVNLPVSLEDLFVGK KKSFKIGRKGPHGASEKTQI DIQLKPGWKAGTKITYKNQG DYNPQTGRRKTLQFVIQEKS HPNFKRDGDDLIYTLPLSFK ESLLGFSKTIQTIDGRTLPL SRVQPVQPSQTSTYPGQGMP TPKNPSQRGNLIVKYKVD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	138
1H chemical shifts	138

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sis1_SBD	1

Entities:

Entity 1, Sis1_SBD 158 residues - Formula weight is not available

1

GLU

THR

VAL

GLN

VAL

ASN

LEU

PRO

2

VAL

SER

LEU

GLU

ASP

LEU

PHE

VAL

GLY

LYS

3

LYS

SER

PHE

LYS

ILE

GLY

ARG

LYS

GLY

4

PRO

HIS

GLY

ALA

SER

GLU

LYS

THR

GLN

ILE

5

ASP

ILE

GLN

LEU

LYS

PRO

GLY

TRP

LYS

ALA

6

GLY

THR

LYS

ILE

THR

TYR

LYS

ASN

GLN

GLY

7

ASP

TYR

ASN

PRO

GLN

THR

GLY

ARG

LYS

8

THR

LEU

GLN

PHE

VAL

ILE

GLN

GLU

LYS

SER

9

HIS

PRO

ASN

PHE

LYS

ARG

ASP

GLY

ASP

10

LEU

ILE

TYR

THR

LEU

PRO

LEU

SER

PHE

LYS

11

GLU

SER

LEU

GLY

PHE

SER

LYS

THR

ILE

12

GLN

THR

ILE

ASP

GLY

ARG

THR

LEU

PRO

LEU

13

SER

ARG

VAL

GLN

PRO

VAL

GLN

PRO

SER

GLN

14

THR

SER

THR

TYR

PRO

GLY

GLN

GLY

MET

PRO

15

THR

PRO

LYS

ASN

PRO

SER

GLN

ARG

GLY

ASN

16

LEU

ILE

VAL

LYS

TYR

LYS

VAL

ASP

Samples:

sample_1: Sis1_SBD, [U-100% 13C; U-100% 15N], 700 uM; potassium chloride 75 mM; potassium phosphate 20 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks