BMRB Entry 27992

Name: MYB28 116-197 chemical shifts
Published: 2019-10-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27992

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

MYB28 116-197 chemical shifts

Deposition date:

2019-08-02

Original release date:

2019-10-29

Authors:

Millard, Peter; Bugge, Katrine; Kragelund, Birthe

Citation:

Citation: Millard, Peter; Bugge, Katrine; Marabini, Riccardo; Boomsma, Wouter; Burow, Meike; Kragelund, Birthe. "IDDomainSpotter: Compositional bias reveals domains in long disordered protein regions - insights from transcription factors" Protein Sci. 29, 169-183 (2020).
PubMed: 31642121

Assembly members:

Assembly members:
MYB28_residues_116-197, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MYB28_residues_116-197: SMEQGIDPVTHKPLASSSNP TVDENLNSPNASSSDKQYSR SSSMPFLSRPPPSSCNMVSK VSELSSNDGTPIQGSSLSCK KR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	190
15N chemical shifts	63
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MYB28 residues 116-197	1

Entities:

Entity 1, MYB28 residues 116-197 82 residues - Formula weight is not available

1

SER

MET

GLU

GLN

GLY

ILE

ASP

PRO

VAL

THR

2

HIS

LYS

PRO

LEU

ALA

SER

ASN

PRO

3

THR

VAL

ASP

GLU

ASN

LEU

ASN

SER

PRO

ASN

4

ALA

SER

ASP

LYS

GLN

TYR

SER

ARG

5

SER

MET

PRO

PHE

LEU

SER

ARG

PRO

6

PRO

SER

CYS

ASN

MET

VAL

SER

LYS

7

VAL

SER

GLU

LEU

SER

ASN

ASP

GLY

THR

8

PRO

ILE

GLN

GLY

SER

LEU

SER

CYS

LYS

9

LYS

ARG

Samples:

sample_1_MYB28_116-197: DSS 1 mM; TCEP 100 uM; sodium azide 0.03 % v/v; sodium phosphate 50 mM; MYB28 residues 116-197, [U-13C; U-15N], 350 uM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1_MYB28_116-197	isotropic	sample_conditions_1
3D HNCACB	sample_1_MYB28_116-197	isotropic	sample_conditions_1
3D HNCOCACB	sample_1_MYB28_116-197	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1_MYB28_116-197	isotropic	sample_conditions_1
3D HNCO	sample_1_MYB28_116-197	isotropic	sample_conditions_1
HN(CA)NNH	sample_1_MYB28_116-197	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - processing

CcpNmr, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks