BMRB Entry 27991

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27991

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Title:
Assignment of 1H, 13C and 15N resonances of Influenza A virus (H1N1) Non structural protein 2 (NS2)
Deposition date:
2019-07-30
Original release date:
2021-07-20
Authors:
Jaiswal, Nancy; Kumar, Dinesh
Citation:

Citation: Jaiswal, Nancy; Agarwal, Nipanshu; Poluri, Krishna Mohan; Kumar, Dinesh. "Effect of urea concentration on instant refolding of Nuclear Export Protein (NEP) from Influenza-A virus H1N1: A solution NMR based investigation"  Int. J. Biol. Macromol. 165, 2508-2519 (2020).
PubMed: 33470198

Assembly members:

Assembly members:
H1N1-NS2, polymer, 119 residues, 14322 Da.

Natural source:

Natural source:   Common Name: Influenza A   Taxonomy ID: 11320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Alphainfluenzavirus Influenza A

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H1N1-NS2: MDSNTMSSFQDILMRMSKMQ LGSSSEDLNGMVTRFESLKI YRDSLGETVMRMGDLHYLQS RNEKWREQLGQKFEEIRWLI EEMRHRLKPTENSFEQITFM QALQLLLEVEQEIRTFSFQ

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts118
1H chemical shifts503

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1H1N1-NS21

Entities:

Entity 1, H1N1-NS2 119 residues - 14322 Da.

protein has 6XHis tag with TEV cleavage site at N terminus

1   METASPSERASNTHRMETSERSERPHEGLN
2   ASPILELEUMETARGMETSERLYSMETGLN
3   LEUGLYSERSERSERGLUASPLEUASNGLY
4   METVALTHRARGPHEGLUSERLEULYSILE
5   TYRARGASPSERLEUGLYGLUTHRVALMET
6   ARGMETGLYASPLEUHISTYRLEUGLNSER
7   ARGASNGLULYSTRPARGGLUGLNLEUGLY
8   GLNLYSPHEGLUGLUILEARGTRPLEUILE
9   GLUGLUMETARGHISARGLEULYSPROTHR
10   GLUASNSERPHEGLUGLNILETHRPHEMET
11   GLNALALEUGLNLEULEULEUGLUVALGLU
12   GLNGLUILEARGTHRPHESERPHEGLN

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