BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27988

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for C-terminal tail of human CRY1

Deposition date: 2019-07-24 Original release date: 2020-10-15

Authors: Lee, Hsiau-Wei; Parico, Gian Carlo; Partch, Carrie

Citation: Parico, Gian Carlo; Lee, Hsiau-Wei; Partch, Carrie. "The CRY1 tail controls circadian timing by regulating its association with CLOCK:BMAL1"  .

Assembly members:
CRY1, polymer, 98 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):
CRY1: gamdpefGLGLLASVPSNPN GNGGFMGYSAENIPGCSSSG SCSQGSGILHYAHGDSQQTH LLKQGRSSMGTGLSGGKRPS QEEDTQSIGPKVQRQSTN

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts96
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRY11

Entities:

Entity 1, CRY1 98 residues - Formula weight is not available

1   GLYALAMETASPPROGLUPHEGLYLEUGLY
2   LEULEUALASERVALPROSERASNPROASN
3   GLYASNGLYGLYPHEMETGLYTYRSERALA
4   GLUASNILEPROGLYCYSSERSERSERGLY
5   SERCYSSERGLNGLYSERGLYILELEUHIS
6   TYRALAHISGLYASPSERGLNGLNTHRHIS
7   LEULEULYSGLNGLYARGSERSERMETGLY
8   THRGLYLEUSERGLYGLYLYSARGPROSER
9   GLNGLUGLUASPTHRGLNSERILEGLYPRO
10   LYSVALGLNARGGLNSERTHRASN

Samples:

sample_1: CRY1, [U-100% 13C; U-100% 15N], 120 uM; MES 20 mM; sodium chloride 100 mM; EDTA 1 mM; TCEP 4 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D (HACA)N(CA)CONsample_1isotropicsample_conditions_1
3D (HACA)N(CA)NCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

CccpNMR v2.4.2, CCPN - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts