BMRB Entry 27986

Name: Mouse CCL2
Published: 2020-09-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27986

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mouse CCL2

Deposition date:

2019-07-24

Original release date:

2020-09-28

Authors:

Joshi, Nidhi; Kumar, Dinesh; Poluri, Krishna Mohan

Citation:

Citation: Joshi, Nidhi; Nagar, Nupur; Gulati, Khushboo; Gangele, Krishnakant; Mishra, Amit; Kumar, Dinesh; Poluri, Krishna Mohan. "Dissecting the differential structural and dynamics features of CCL2 chemokine orthologs" Int. J. Biol. Macromol. 156, 239-251 (2020).
PubMed: 32289428

Assembly members:

Assembly members:
CCL2, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CCL2: QPDAVNAPLTCCYSFTSKMI PMSRLESYKRITSSRCPKEA VVFVTKLKREVCADPKKEWV QTYIKNLDRNQMRSEP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	140
15N chemical shifts	70
1H chemical shifts	136

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CCL2	1

Entities:

Entity 1, CCL2 76 residues - Formula weight is not available

1

GLN

PRO

ASP

ALA

VAL

ASN

ALA

PRO

LEU

THR

2

CYS

TYR

SER

PHE

THR

SER

LYS

MET

ILE

3

PRO

MET

SER

ARG

LEU

GLU

SER

TYR

LYS

ARG

4

ILE

THR

SER

ARG

CYS

PRO

LYS

GLU

ALA

5

VAL

PHE

VAL

THR

LYS

LEU

LYS

ARG

GLU

6

VAL

CYS

ALA

ASP

PRO

LYS

GLU

TRP

VAL

7

GLN

THR

TYR

ILE

LYS

ASN

LEU

ASP

ARG

ASN

8

GLN

MET

ARG

SER

GLU

PRO

Samples:

sample_1: CCL2, [U-99% 13C; U-99% 15N], 1.0 mM; D2O, [U-100% 2H], 10%; H2O 90%; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 800 MHz