BMRB Entry 27975

Name: Mouse Lemur Biliverdin B reductase
Published: 2020-09-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27975

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mouse Lemur Biliverdin B reductase

Deposition date:

2019-07-16

Original release date:

2020-09-28

Authors:

Eisenmesser, Elan; Redzic, Jasmina; Paukovich, Natasia

Citation:

Citation: Duff, Michael; Redzic, Jasmina; Ryan, Lucas; Paukovich, Natasia; Zhao, Rui; Nix, Jay; Pitts, Todd; Agarwal, Pratul; Eisenmesser, Elan Zohar. "Structure, dynamics and function of the evolutionarily changing biliverdin reductase B family" J. Biochem. 168, 191-202 (2020).
PubMed: 32246827

Assembly members:

Assembly members:
Mouse_Lemur_BLVRB, polymer, 220 residues, Formula weight is not available

Natural source:

Natural source: Common Name: gray mouse lemur Taxonomy ID: 30608 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Microcebus murinus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mouse_Lemur_BLVRB: MGHHHHHHLVPRGSMAVKKI VIFGATGRTGLTTLAQAVQA GYEVTVLVRDSSRLPSEGPR PARVIVGDVRHAADVDKAVA GQDAVVVLLGTGNDLSPTTV MSEGARNIVAAMKAHGVDKV VACTSAFLLWDPAKVPPRLQ DVTDDHIRMHKVLQESGLKY VAVMPPHIGDQPLTGAYSVT LDGRGPSRVISKHDLGHFML HCLTTAEYDGHSTYPSHQYD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	331
15N chemical shifts	178
1H chemical shifts	178

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mouse Lemur BLVRB monomer	1

Entities:

Entity 1, Mouse Lemur BLVRB monomer 220 residues - Formula weight is not available

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	LEU	VAL
2	PRO	ARG	GLY	SER	MET	ALA	VAL	LYS	LYS	ILE
3	VAL	ILE	PHE	GLY	ALA	THR	GLY	ARG	THR	GLY
4	LEU	THR	THR	LEU	ALA	GLN	ALA	VAL	GLN	ALA
5	GLY	TYR	GLU	VAL	THR	VAL	LEU	VAL	ARG	ASP
6	SER	SER	ARG	LEU	PRO	SER	GLU	GLY	PRO	ARG
7	PRO	ALA	ARG	VAL	ILE	VAL	GLY	ASP	VAL	ARG
8	HIS	ALA	ALA	ASP	VAL	ASP	LYS	ALA	VAL	ALA
9	GLY	GLN	ASP	ALA	VAL	VAL	VAL	LEU	LEU	GLY
10	THR	GLY	ASN	ASP	LEU	SER	PRO	THR	THR	VAL
11	MET	SER	GLU	GLY	ALA	ARG	ASN	ILE	VAL	ALA
12	ALA	MET	LYS	ALA	HIS	GLY	VAL	ASP	LYS	VAL
13	VAL	ALA	CYS	THR	SER	ALA	PHE	LEU	LEU	TRP
14	ASP	PRO	ALA	LYS	VAL	PRO	PRO	ARG	LEU	GLN
15	ASP	VAL	THR	ASP	ASP	HIS	ILE	ARG	MET	HIS
16	LYS	VAL	LEU	GLN	GLU	SER	GLY	LEU	LYS	TYR
17	VAL	ALA	VAL	MET	PRO	PRO	HIS	ILE	GLY	ASP
18	GLN	PRO	LEU	THR	GLY	ALA	TYR	SER	VAL	THR
19	LEU	ASP	GLY	ARG	GLY	PRO	SER	ARG	VAL	ILE
20	SER	LYS	HIS	ASP	LEU	GLY	HIS	PHE	MET	LEU
21	HIS	CYS	LEU	THR	THR	ALA	GLU	TYR	ASP	GLY
22	HIS	SER	THR	TYR	PRO	SER	HIS	GLN	TYR	ASP

Samples:

sample_1: Mouse Lemur BLVRB, [U-15N], 0.5 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 20 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz
Varian DRX 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	LEU	VAL
2	PRO	ARG	GLY	SER	MET	ALA	VAL	LYS	LYS	ILE
3	VAL	ILE	PHE	GLY	ALA	THR	GLY	ARG	THR	GLY
4	LEU	THR	THR	LEU	ALA	GLN	ALA	VAL	GLN	ALA
5	GLY	TYR	GLU	VAL	THR	VAL	LEU	VAL	ARG	ASP
6	SER	SER	ARG	LEU	PRO	SER	GLU	GLY	PRO	ARG
7	PRO	ALA	ARG	VAL	ILE	VAL	GLY	ASP	VAL	ARG
8	HIS	ALA	ALA	ASP	VAL	ASP	LYS	ALA	VAL	ALA
9	GLY	GLN	ASP	ALA	VAL	VAL	VAL	LEU	LEU	GLY
10	THR	GLY	ASN	ASP	LEU	SER	PRO	THR	THR	VAL
11	MET	SER	GLU	GLY	ALA	ARG	ASN	ILE	VAL	ALA
12	ALA	MET	LYS	ALA	HIS	GLY	VAL	ASP	LYS	VAL
13	VAL	ALA	CYS	THR	SER	ALA	PHE	LEU	LEU	TRP
14	ASP	PRO	ALA	LYS	VAL	PRO	PRO	ARG	LEU	GLN
15	ASP	VAL	THR	ASP	ASP	HIS	ILE	ARG	MET	HIS
16	LYS	VAL	LEU	GLN	GLU	SER	GLY	LEU	LYS	TYR
17	VAL	ALA	VAL	MET	PRO	PRO	HIS	ILE	GLY	ASP
18	GLN	PRO	LEU	THR	GLY	ALA	TYR	SER	VAL	THR
19	LEU	ASP	GLY	ARG	GLY	PRO	SER	ARG	VAL	ILE
20	SER	LYS	HIS	ASP	LEU	GLY	HIS	PHE	MET	LEU
21	HIS	CYS	LEU	THR	THR	ALA	GLU	TYR	ASP	GLY
22	HIS	SER	THR	TYR	PRO	SER	HIS	GLN	TYR	ASP

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	LEU	VAL
2	PRO	ARG	GLY	SER	MET	ALA	VAL	LYS	LYS	ILE
3	VAL	ILE	PHE	GLY	ALA	THR	GLY	ARG	THR	GLY
4	LEU	THR	THR	LEU	ALA	GLN	ALA	VAL	GLN	ALA
5	GLY	TYR	GLU	VAL	THR	VAL	LEU	VAL	ARG	ASP
6	SER	SER	ARG	LEU	PRO	SER	GLU	GLY	PRO	ARG
7	PRO	ALA	ARG	VAL	ILE	VAL	GLY	ASP	VAL	ARG
8	HIS	ALA	ALA	ASP	VAL	ASP	LYS	ALA	VAL	ALA
9	GLY	GLN	ASP	ALA	VAL	VAL	VAL	LEU	LEU	GLY
10	THR	GLY	ASN	ASP	LEU	SER	PRO	THR	THR	VAL
11	MET	SER	GLU	GLY	ALA	ARG	ASN	ILE	VAL	ALA
12	ALA	MET	LYS	ALA	HIS	GLY	VAL	ASP	LYS	VAL
13	VAL	ALA	CYS	THR	SER	ALA	PHE	LEU	LEU	TRP
14	ASP	PRO	ALA	LYS	VAL	PRO	PRO	ARG	LEU	GLN
15	ASP	VAL	THR	ASP	ASP	HIS	ILE	ARG	MET	HIS
16	LYS	VAL	LEU	GLN	GLU	SER	GLY	LEU	LYS	TYR
17	VAL	ALA	VAL	MET	PRO	PRO	HIS	ILE	GLY	ASP
18	GLN	PRO	LEU	THR	GLY	ALA	TYR	SER	VAL	THR
19	LEU	ASP	GLY	ARG	GLY	PRO	SER	ARG	VAL	ILE
20	SER	LYS	HIS	ASP	LEU	GLY	HIS	PHE	MET	LEU
21	HIS	CYS	LEU	THR	THR	ALA	GLU	TYR	ASP	GLY
22	HIS	SER	THR	TYR	PRO	SER	HIS	GLN	TYR	ASP

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	LEU	VAL
2	PRO	ARG	GLY	SER	MET	ALA	VAL	LYS	LYS	ILE
3	VAL	ILE	PHE	GLY	ALA	THR	GLY	ARG	THR	GLY
4	LEU	THR	THR	LEU	ALA	GLN	ALA	VAL	GLN	ALA
5	GLY	TYR	GLU	VAL	THR	VAL	LEU	VAL	ARG	ASP
6	SER	SER	ARG	LEU	PRO	SER	GLU	GLY	PRO	ARG
7	PRO	ALA	ARG	VAL	ILE	VAL	GLY	ASP	VAL	ARG
8	HIS	ALA	ALA	ASP	VAL	ASP	LYS	ALA	VAL	ALA
9	GLY	GLN	ASP	ALA	VAL	VAL	VAL	LEU	LEU	GLY
10	THR	GLY	ASN	ASP	LEU	SER	PRO	THR	THR	VAL
11	MET	SER	GLU	GLY	ALA	ARG	ASN	ILE	VAL	ALA
12	ALA	MET	LYS	ALA	HIS	GLY	VAL	ASP	LYS	VAL
13	VAL	ALA	CYS	THR	SER	ALA	PHE	LEU	LEU	TRP
14	ASP	PRO	ALA	LYS	VAL	PRO	PRO	ARG	LEU	GLN
15	ASP	VAL	THR	ASP	ASP	HIS	ILE	ARG	MET	HIS
16	LYS	VAL	LEU	GLN	GLU	SER	GLY	LEU	LYS	TYR
17	VAL	ALA	VAL	MET	PRO	PRO	HIS	ILE	GLY	ASP
18	GLN	PRO	LEU	THR	GLY	ALA	TYR	SER	VAL	THR
19	LEU	ASP	GLY	ARG	GLY	PRO	SER	ARG	VAL	ILE
20	SER	LYS	HIS	ASP	LEU	GLY	HIS	PHE	MET	LEU
21	HIS	CYS	LEU	THR	THR	ALA	GLU	TYR	ASP	GLY
22	HIS	SER	THR	TYR	PRO	SER	HIS	GLN	TYR	ASP