BMRB Entry 27972
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27972
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NMR-STAR v3 text file.
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Citation: Gould, Joseph; Qiu, Shihong; Shang, Qiao; Ogino, Tomoaki; Prevelige, Peter; Petit, Chad; Green, Todd. "The Connector Domain of Vesicular Stomatitis Virus Large Protein Interacts with the Viral Phosphoprotein" J. Virol. 94, e01729-19-e01729-19 (2020).
PubMed: 31896592
Assembly members:
L_Protein, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: Vesicular Stomatitis Virus Taxonomy ID: 11276 Superkingdom: Viruses Kingdom: not available Genus/species: Vesiculovirus Vesicular Stomatitis Virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: SpGFTk
Entity Sequences (FASTA):
L_Protein: SNYELFQEDGVEEHTRPSYF
QAADDSDTESEPEIEDNQGL
YVPDPEAEQVEGFIQGPLDD
YADEDVDVVFTS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 114 |
| 15N chemical shifts | 60 |
| 1H chemical shifts | 60 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VSV Phosphoprotein 35-106 | 1 |
Entities:
Entity 1, VSV Phosphoprotein 35-106 72 residues - Formula weight is not available
| 1 | SER | ASN | TYR | GLU | LEU | PHE | GLN | GLU | ASP | GLY | ||||
| 2 | VAL | GLU | GLU | HIS | THR | ARG | PRO | SER | TYR | PHE | ||||
| 3 | GLN | ALA | ALA | ASP | ASP | SER | ASP | THR | GLU | SER | ||||
| 4 | GLU | PRO | GLU | ILE | GLU | ASP | ASN | GLN | GLY | LEU | ||||
| 5 | TYR | VAL | PRO | ASP | PRO | GLU | ALA | GLU | GLN | VAL | ||||
| 6 | GLU | GLY | PHE | ILE | GLN | GLY | PRO | LEU | ASP | ASP | ||||
| 7 | TYR | ALA | ASP | GLU | ASP | VAL | ASP | VAL | VAL | PHE | ||||
| 8 | THR | SER |
Samples:
sample_1: VSV Phosphoprotein 35-106, [U-13C; U-15N], 1 mM; HEPES 20 mM; Sodium Chloride 150 mM; glycerol 10%; TCEP 1 mM; sodium azide 0.02%
sample_conditions_1: ionic strength: 0.150 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks