BMRB Entry 27970

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27970

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Title:
ILV(proS) methyl assignment of mIL-2 in complex with JES6-1 scFV antibody
Deposition date:
2019-07-11
Original release date:
2020-04-03
Authors:
De Paula, Viviane; Sgourakis, Nikolaos
Citation:

Citation: De Paula, Viviane; Jude, Kevin; Nerli, Santrupti; Glassman, Caleb; Garcia, K Christopher; Sgourakis, Nikolaos. "Interleukin-2 druggability is modulated by global conformational transitions controlled by a helical capping switch"  Proc. Natl. Acad. Sci. U.S.A. 117, 7183-7192 (2020).
PubMed: 32184322

Assembly members:

Assembly members:
mIL-2/JES6-1_complex, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mIL-2/JES6-1_complex: APTSSSTSSSTAEAQQQQQQ QQQQQQHLEQLLMDLQELLS RMENYRNLKLPRMLTFKFYL PKQATELKDLQCLEDELGPL RHVLDLTQSKSFQLEDAENF ISNIRVTVVKLKGSDNTFEC QFDDESATVVDFLRRWIAFC QSIISTSPQAAAHHHHHHHH

Data sets:
Data typeCount
13C chemical shifts25
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mIL-2/JES6-1 complex1

Entities:

Entity 1, mIL-2/JES6-1 complex 160 residues - Formula weight is not available

1   ALAPROTHRSERSERSERTHRSERSERSER
2   THRALAGLUALAGLNGLNGLNGLNGLNGLN
3   GLNGLNGLNGLNGLNGLNHISLEUGLUGLN
4   LEULEUMETASPLEUGLNGLULEULEUSER
5   ARGMETGLUASNTYRARGASNLEULYSLEU
6   PROARGMETLEUTHRPHELYSPHETYRLEU
7   PROLYSGLNALATHRGLULEULYSASPLEU
8   GLNCYSLEUGLUASPGLULEUGLYPROLEU
9   ARGHISVALLEUASPLEUTHRGLNSERLYS
10   SERPHEGLNLEUGLUASPALAGLUASNPHE
11   ILESERASNILEARGVALTHRVALVALLYS
12   LEULYSGLYSERASPASNTHRPHEGLUCYS
13   GLNPHEASPASPGLUSERALATHRVALVAL
14   ASPPHELEUARGARGTRPILEALAPHECYS
15   GLNSERILEILESERTHRSERPROGLNALA
16   ALAALAHISHISHISHISHISHISHISHIS

Samples:

sample_1: mIL-2/JES6-1 complex, [U-13C; U-15N; U-2H; ILV(proS) methyl], 200 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQC methylsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphatic (CCH)sample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphatic (HCH)sample_1isotropicsample_conditions_1

Software:

CCPN, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 800 MHz