BMRB Entry 27968

Name: Solution NMR Backbone Resonance Assignments for p150Glued(1-191) of Dynactin
Published: 2019-07-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27968

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Backbone Resonance Assignments for p150Glued(1-191) of Dynactin

Deposition date:

2019-07-05

Original release date:

2019-07-12

Authors:

Guo, Changmiao; Polenova, Tatyana

Citation:

Citation: Guo, Changmiao; Williams, John; Polenova, Tatyana. "Conformational Flexibility of p150Glued(1-191) Subunit of Dynactin Assembled with Microtubules" Biophys. J. 117, 938-949 (2019).
PubMed: 31445682

Assembly members:

Assembly members:
Extended_CAP-Gly, polymer, 191 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Extended_CAP-Gly: MAQSKRHMYNRTPSGSRMST EASARPLRVGSRVEVIGKGH RGTVAYVGATLFATGKWVGV ILDEAKGKNDGTVQGRKYFT CDEGHGIFVRQSQIQVFEDG ADTTSPETPDSSASKILKRE GADAAAKTSKLRGLKPKKAP TARKTTTRRPKPTRPASTGV AGPSSSLGPSGSASAGELSS SEPSTPAQTPL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	191
15N chemical shifts	176
1H chemical shifts	176

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p150Glued(1-191)	1

Entities:

Entity 1, p150Glued(1-191) 191 residues - Formula weight is not available

1

MET

ALA

GLN

SER

LYS

ARG

HIS

MET

TYR

ASN

2

ARG

THR

PRO

SER

GLY

SER

ARG

MET

SER

THR

3

GLU

ALA

SER

ALA

ARG

PRO

LEU

ARG

VAL

GLY

4

SER

ARG

VAL

GLU

VAL

ILE

GLY

LYS

GLY

HIS

5

ARG

GLY

THR

VAL

ALA

TYR

VAL

GLY

ALA

THR

6

LEU

PHE

ALA

THR

GLY

LYS

TRP

VAL

GLY

VAL

7

ILE

LEU

ASP

GLU

ALA

LYS

GLY

LYS

ASN

ASP

8

GLY

THR

VAL

GLN

GLY

ARG

LYS

TYR

PHE

THR

9

CYS

ASP

GLU

GLY

HIS

GLY

ILE

PHE

VAL

ARG

10

GLN

SER

GLN

ILE

GLN

VAL

PHE

GLU

ASP

GLY

11

ALA

ASP

THR

SER

PRO

GLU

THR

PRO

ASP

12

SER

ALA

SER

LYS

ILE

LEU

LYS

ARG

GLU

13

GLY

ALA

ASP

ALA

LYS

THR

SER

LYS

14

LEU

ARG

GLY

LEU

LYS

PRO

LYS

ALA

PRO

15

THR

ALA

ARG

LYS

THR

ARG

PRO

16

LYS

PRO

THR

ARG

PRO

ALA

SER

THR

GLY

VAL

17

ALA

GLY

PRO

SER

LEU

GLY

PRO

SER

18

GLY

SER

ALA

SER

ALA

GLY

GLU

LEU

SER

19

SER

GLU

PRO

SER

THR

PRO

ALA

GLN

THR

PRO

20

LEU

Samples:

sample_1: Extended CAP-Gly, [U-99% 13C; U-99% 15N], 300 uM; phosphate 20 mM; NaCl 50 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis, Skinner, Goult, Fogh, Boucher, Stevens, Laue and Vuister - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks