BMRB Entry 27963

Name: RDC of Delta subunit of RNA polymerase from Bacillus subtilis
Published: 2019-09-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27963

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RDC of Delta subunit of RNA polymerase from Bacillus subtilis

Deposition date:

2019-06-30

Original release date:

2019-09-30

Authors:

Zidek, Lukas; Kuban, Vojtech; Srb, Pavel; Stegnerova, Hana; Padrta, Petr; Zachrdla, Milan; Jasenakova, Zuzana; Sanderova, Hana; Krasny, Libor

Citation:

Citation: Kuban, Vojtech; Srb, Pavel; Stegnerova, Hana; Padrta, Petr; Zachrdla, Milan; Jasenakova, Zuzana; Sanderova, Hana; Vitovska, Dragana; Krasny, Libor; Koval, Tomas; Dohnalek, Jan; Ziemska-Legi Cka, Joanna; Grynberg, Marcin; Jarnot, Patryk; Gruca, Aleksandra; Jensen, Malene Ringkjobing; Blackledge, Martin; Zidek, Lukas. "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase" J. Am. Chem. Soc. 141, 16817-16828 (2019).
PubMed: 31550880

Assembly members:

Assembly members:
Delta_Subunit, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Delta_Subunit: MGIKQYSQEELKEMALVEIA HELFEEHKKPVPFQELLNEI ASLLGVKKEELGDRIAQFYT DLNIDGRFLALSDQTWGLRS WYPYDQLDEETQPTVKAKKK KAKKAVEEDLDLDEFEEIDE DDLDLDEVEEELDLEADDFD EEDLDEDDDDLEIEEDIIDE DDEDYDDEEEEIK

Data sets:

RDCs
- RDC_list_1

Data type	Count
residual dipolar couplings	481

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Delta	1

Entities:

Entity 1, Delta 173 residues - Formula weight is not available

1

MET

GLY

ILE

LYS

GLN

TYR

SER

GLN

GLU

2

LEU

LYS

GLU

MET

ALA

LEU

VAL

GLU

ILE

ALA

3

HIS

GLU

LEU

PHE

GLU

HIS

LYS

PRO

4

VAL

PRO

PHE

GLN

GLU

LEU

ASN

GLU

ILE

5

ALA

SER

LEU

GLY

VAL

LYS

GLU

6

LEU

GLY

ASP

ARG

ILE

ALA

GLN

PHE

TYR

THR

7

ASP

LEU

ASN

ILE

ASP

GLY

ARG

PHE

LEU

ALA

8

LEU

SER

ASP

GLN

THR

TRP

GLY

LEU

ARG

SER

9

TRP

TYR

PRO

TYR

ASP

GLN

LEU

ASP

GLU

10

THR

GLN

PRO

THR

VAL

LYS

ALA

LYS

11

LYS

ALA

LYS

ALA

VAL

GLU

ASP

LEU

12

ASP

LEU

ASP

GLU

PHE

GLU

ILE

ASP

GLU

13

ASP

LEU

ASP

LEU

ASP

GLU

VAL

GLU

14

GLU

LEU

ASP

LEU

GLU

ALA

ASP

PHE

ASP

15

GLU

ASP

LEU

ASP

GLU

ASP

16

LEU

GLU

ILE

GLU

ASP

ILE

ASP

GLU

17

ASP

GLU

ASP

TYR

ASP

GLU

18

GLU

ILE

LYS

Samples:

sample_gel: Delta Subunit of Bacterial RNA Polymerase, [U-100% 13C; U-100% 15N], 0.6 mM; sodium chloride 10 mM; sodium phosphate 20 mM; sodium azide 0.05%

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D IPAP HNCO	sample_gel	anisotropic	sample_conditions_1
3D a/b-HNCO	sample_gel	anisotropic	sample_conditions_1
4D IPAP (HA)CACONH	sample_gel	anisotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz