BMRB Entry 27962

Name: 1H, 15N, and 13C chemical shifts of red abalone FITZAP-8D
Published: 2020-02-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27962

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, and 13C chemical shifts of red abalone FITZAP-8D

Deposition date:

2019-06-28

Original release date:

2020-02-26

Authors:

Wilburn, Damien; Tuttle, Lisa; Klevit, Rachel; Swanson, Willie

Citation:

Citation: Wilburn, Damien; Tuttle, Lisa; Klevit, Rachel; Swanson, Willie. "Indirect sexual selection drives rapid sperm protein evolution in abalone" Elife 8, e52628-e52628 (2019).
PubMed: 31868593

Assembly members:

Assembly members:
FITZAP-8D, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Red abalone Taxonomy ID: 6454 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Haliotis rufescens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FITZAP-8D: GSHGRTSSYDDDDDDDDEPP KDLITKDVIEAVGGNIKMFQ RRKRSADLDYGGRYGNDDPD SYLE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	77
15N chemical shifts	26
1H chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FITZAP-8D	1

Entities:

Entity 1, FITZAP-8D 64 residues - Formula weight is not available

1

GLY

SER

HIS

GLY

ARG

THR

SER

TYR

ASP

2

ASP

GLU

PRO

3

LYS

ASP

LEU

ILE

THR

LYS

ASP

VAL

ILE

GLU

4

ALA

VAL

GLY

ASN

ILE

LYS

MET

PHE

GLN

5

ARG

LYS

ARG

SER

ALA

ASP

LEU

ASP

TYR

6

GLY

ARG

TYR

GLY

ASN

ASP

PRO

ASP

7

SER

TYR

LEU

GLU

Samples:

rFITZAP-8D: FITZAP-8D+ATCUN, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	rFITZAP-8D	isotropic	sample_conditions_1
2D 1H-13C HSQC	rFITZAP-8D	isotropic	sample_conditions_1
3D CBCA(CO)NH	rFITZAP-8D	isotropic	sample_conditions_1
3D HNCO	rFITZAP-8D	isotropic	sample_conditions_1
3D HNCACB	rFITZAP-8D	isotropic	sample_conditions_1
3D 1H-15N NOESY	rFITZAP-8D	isotropic	sample_conditions_1
3D 1H-15N TOCSY	rFITZAP-8D	isotropic	sample_conditions_1
3D HNHA	rFITZAP-8D	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks