BMRB Entry 27959

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27959

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Title:
Including protons in solid-state NMR resonance assignment and secondary structure analysis: The example of RNA polymerase II subunits Rpo4/7
Deposition date:
2019-06-25
Original release date:
2019-07-23
Authors:
Torosyan, Anahit; Wiegand, Thomas; Schledorn, Maarten; Boeckmann, Anja; Meier, Beat
Citation:

Citation: Torosyan, Anahit; Wiegand, Thomas; Schledorn, Maarten; Klose, Daniel; Guentert, Peter; Boeckmann, Anja; Meier, Beat. "Including protons in solid-state NMR resonance assignment and secondary structure analysis: The example of RNA polymerase II subunits Rpo4/7"  Front. Mol. Biosci. 6, 100-100 (2019).
PubMed: 31637245

Assembly members:

Assembly members:
Rpo7, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Methanococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21_Rpo7S65C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rpo7: MYKILEIADVVKVPPEEFGK DLKETVKKILMEKYEGRLDK DVGFVLSIVDVKDIGEGKVV HGDGSAYHPVVFETLVYIPE MYELIEGEVVDVVEFGSFVR LGPLDGLIHVSQIMDDYVSY DPKREAIIGKETGKVLEIGD YVRARIVAISLKAERKRGSK IALTMRQPYLGKLEWIEEEK AKKQNQE

Data sets:
Data typeCount
13C chemical shifts594
15N chemical shifts145
1H chemical shifts265

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rpo71

Entities:

Entity 1, Rpo7 187 residues - Formula weight is not available

1   METTYRLYSILELEUGLUILEALAASPVAL
2   VALLYSVALPROPROGLUGLUPHEGLYLYS
3   ASPLEULYSGLUTHRVALLYSLYSILELEU
4   METGLULYSTYRGLUGLYARGLEUASPLYS
5   ASPVALGLYPHEVALLEUSERILEVALASP
6   VALLYSASPILEGLYGLUGLYLYSVALVAL
7   HISGLYASPGLYSERALATYRHISPROVAL
8   VALPHEGLUTHRLEUVALTYRILEPROGLU
9   METTYRGLULEUILEGLUGLYGLUVALVAL
10   ASPVALVALGLUPHEGLYSERPHEVALARG
11   LEUGLYPROLEUASPGLYLEUILEHISVAL
12   SERGLNILEMETASPASPTYRVALSERTYR
13   ASPPROLYSARGGLUALAILEILEGLYLYS
14   GLUTHRGLYLYSVALLEUGLUILEGLYASP
15   TYRVALARGALAARGILEVALALAILESER
16   LEULYSALAGLUARGLYSARGGLYSERLYS
17   ILEALALEUTHRMETARGGLNPROTYRLEU
18   GLYLYSLEUGLUTRPILEGLUGLUGLULYS
19   ALALYSLYSGLNASNGLNGLU

Samples:

sample_1: RNA polymerase subunit Rpo7, [U-100% 13C; U-100% 15N], 16 mg; RNA polymerase subunit Rpo4 9 mg

sample_conditions_1: ionic strength: 130 mM; pH: 7.9; pressure: 1 atm; temperature: 273 K

sample_conditions_2: ionic strength: 130 mM; pH: 7.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D NCACBsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D CANCOsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D NcoCACBsample_1isotropicsample_conditions_1
3D CANcoCAsample_1isotropicsample_conditions_1
3D CCCsample_1isotropicsample_conditions_1
2D CCsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
3D hNCAHsample_1isotropicsample_conditions_2
3D hCANHsample_1isotropicsample_conditions_2
3D hCONHsample_1isotropicsample_conditions_2
2D hNHsample_1isotropicsample_conditions_2
2D hCHsample_1isotropicsample_conditions_2

Software:

CCPNmr v2.4.2, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks