BMRB Entry 27958

Name: Spin-labeled DNA duplex
Published: 2020-02-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27958

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Spin-labeled DNA duplex

Deposition date:

2019-06-24

Original release date:

2020-02-26

Authors:

Lane, Andrew

Citation:

Citation: Hardwick, Jack; Haugland, Marius; El-Sagheer, Afaf; Ptchelkine, Denis; Beierlein, Frank; Lane, Andrew; Brown, Tom; Lovett, Janet; Anderson, Edward. "2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis" Nucleic Acids Res. 48, 2830-2840 (2020).
PubMed: 32052020

Assembly members:

Assembly members:
DNA_strand_A, polymer, 14 residues, Formula weight is not available
DNA_strand_B, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_strand_A: CTXACGCGTCATTG
DNA_strand_B: CAATGACGCGTAAG

Data sets:

assigned_chemical_shifts

Data type	Count
1H chemical shifts	618

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNA strand A	1
2	DNA strand B	2

Entities:

Entity 1, DNA strand A 14 residues - Formula weight is not available

1

DC

DT

DX

DA

DC

DG

DC

DG

DT

DC

2

DA

DT

DG

Entity 2, DNA strand B 14 residues - Formula weight is not available

1

DC

DA

DT

DG

DA

DC

DG

DC

DG

2

DT

DA

DG

Samples:

sample_1: DNA strand A 0.75 mM; DNA strand B 0.75 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 30 K

Experiments:

Name	Sample	Sample state	Sample conditions
Presat	sample_1	isotropic	sample_conditions_1
2D 2Q COSY	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1
1D water suppression	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Agilent - data analysis

NMR spectrometers:

Agilent DD2 600 MHz