BMRB Entry 27952

Name: Sequence-specific assignments of the P. falciparum PFE0660w J-domain
Published: 2019-09-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27952

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific assignments of the P. falciparum PFE0660w J-domain

Deposition date:

2019-06-21

Original release date:

2019-09-23

Authors:

Vakonakis, Ioannis; Day, Jemma

Citation:

Citation: Day, Jemma; Passecker, Armin; Beck, Hans-Peter; Vakonakis, Ioannis. "The Plasmodium falciparum Hsp70-x chaperone assists the heat stress response of the malaria parasite" FASEB J. 33, 14611-14624 (2019).
PubMed: 31690116

Assembly members:

Assembly members:
PFA0660w-J, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pFLOAT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PFA0660w-J: GPMDYYTLLGVDKGCSEDDL RRAYLKLAMKWHPDKHVNKG SKVEAEEKFKNICEAYSVLS DNEKRVKYDL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	123
15N chemical shifts	59
1H chemical shifts	59

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	J-domain	1

Entities:

Entity 1, J-domain 70 residues - Formula weight is not available

Two amino acids (GP) from cloning artefact. Remaining comprises amino acids 81-148 of UniProt Q8I2E1.

1	GLY	PRO	MET	ASP	TYR	TYR	THR	LEU	LEU	GLY
2	VAL	ASP	LYS	GLY	CYS	SER	GLU	ASP	ASP	LEU
3	ARG	ARG	ALA	TYR	LEU	LYS	LEU	ALA	MET	LYS
4	TRP	HIS	PRO	ASP	LYS	HIS	VAL	ASN	LYS	GLY
5	SER	LYS	VAL	GLU	ALA	GLU	GLU	LYS	PHE	LYS
6	ASN	ILE	CYS	GLU	ALA	TYR	SER	VAL	LEU	SER
7	ASP	ASN	GLU	LYS	ARG	VAL	LYS	TYR	ASP	LEU

Samples:

sample_1: PFA0660w-J, [U-98% 13C; U-98% 15N], 0.5 mM; D2O 5%; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 2 mM; DSS 0.05 mM

sample_conditions_1: ionic strength: 0.38 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 750 MHz

1	GLY	PRO	MET	ASP	TYR	TYR	THR	LEU	LEU	GLY
2	VAL	ASP	LYS	GLY	CYS	SER	GLU	ASP	ASP	LEU
3	ARG	ARG	ALA	TYR	LEU	LYS	LEU	ALA	MET	LYS
4	TRP	HIS	PRO	ASP	LYS	HIS	VAL	ASN	LYS	GLY
5	SER	LYS	VAL	GLU	ALA	GLU	GLU	LYS	PHE	LYS
6	ASN	ILE	CYS	GLU	ALA	TYR	SER	VAL	LEU	SER
7	ASP	ASN	GLU	LYS	ARG	VAL	LYS	TYR	ASP	LEU

1	GLY	PRO	MET	ASP	TYR	TYR	THR	LEU	LEU	GLY
2	VAL	ASP	LYS	GLY	CYS	SER	GLU	ASP	ASP	LEU
3	ARG	ARG	ALA	TYR	LEU	LYS	LEU	ALA	MET	LYS
4	TRP	HIS	PRO	ASP	LYS	HIS	VAL	ASN	LYS	GLY
5	SER	LYS	VAL	GLU	ALA	GLU	GLU	LYS	PHE	LYS
6	ASN	ILE	CYS	GLU	ALA	TYR	SER	VAL	LEU	SER
7	ASP	ASN	GLU	LYS	ARG	VAL	LYS	TYR	ASP	LEU