BMRB Entry 27950

Name: Backbone assignments of apo Vaccinia H1-Related (VHR) protein tyrosine phosphatase (PTP)
Published: 2019-07-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27950

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of apo Vaccinia H1-Related (VHR) protein tyrosine phosphatase (PTP)

Deposition date:

2019-06-18

Original release date:

2019-07-16

Authors:

Loria, Joseph; Beaumont, Victor

Citation:

Citation: Loria, Joseph; Beaumont, Victor. "Solution state backbone assignments of Vaccinia H1-Related (VHR) protein tyrosine phosphatase (PTP)" The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
Vaccinia_H1-Related_PTP, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet43.1b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Vaccinia_H1-Related_PTP: MSGSFELSVQDLNDLLSDGS GCYSLPSQPCNEVTPRIYVG NASVAQDIPKLQKLGITHVL NAAEGRSFMHVNTNANFYKD SGITYLGIKANDTQEFNLSA YFERAADFIDQALAQKNGRV LVHCREGYSRSPTLVIAYLM MRQKMDVKSALSIVRQNREI GPNDGFLAQLCQLNDRLAKE GKLKP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	473
15N chemical shifts	166
1H chemical shifts	166

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Vaccinia H1-Related PTP (VHR)	1

Entities:

Entity 1, Vaccinia H1-Related PTP (VHR) 185 residues - Formula weight is not available

1

MET

SER

GLY

SER

PHE

GLU

LEU

SER

VAL

GLN

2

ASP

LEU

ASN

ASP

LEU

SER

ASP

GLY

SER

3

GLY

CYS

TYR

SER

LEU

PRO

SER

GLN

PRO

CYS

4

ASN

GLU

VAL

THR

PRO

ARG

ILE

TYR

VAL

GLY

5

ASN

ALA

SER

VAL

ALA

GLN

ASP

ILE

PRO

LYS

6

LEU

GLN

LYS

LEU

GLY

ILE

THR

HIS

VAL

LEU

7

ASN

ALA

GLU

GLY

ARG

SER

PHE

MET

HIS

8

VAL

ASN

THR

ASN

ALA

ASN

PHE

TYR

LYS

ASP

9

SER

GLY

ILE

THR

TYR

LEU

GLY

ILE

LYS

ALA

10

ASN

ASP

THR

GLN

GLU

PHE

ASN

LEU

SER

ALA

11

TYR

PHE

GLU

ARG

ALA

ASP

PHE

ILE

ASP

12

GLN

ALA

LEU

ALA

GLN

LYS

ASN

GLY

ARG

VAL

13

LEU

VAL

HIS

CYS

ARG

GLU

GLY

TYR

SER

ARG

14

SER

PRO

THR

LEU

VAL

ILE

ALA

TYR

LEU

MET

15

MET

ARG

GLN

LYS

MET

ASP

VAL

LYS

SER

ALA

16

LEU

SER

ILE

VAL

ARG

GLN

ASN

ARG

GLU

ILE

17

GLY

PRO

ASN

ASP

GLY

PHE

LEU

ALA

GLN

LEU

18

CYS

GLN

LEU

ASN

ASP

ARG

LEU

ALA

LYS

GLU

19

GLY

LYS

LEU

LYS

PRO

Samples:

sample_1: Vaccinia H1-Related PTP, [U-100% 13C; U-100% 15N], 540 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Varian INOVA 600 MHz
Varian DD2 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks