BMRB Entry 27944

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for ribosomal protein bL12 S89C
Published: 2019-09-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27944

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for ribosomal protein bL12 S89C

Deposition date:

2019-06-16

Original release date:

2019-09-25

Authors:

Wang, Xiaolin; Kirkpatrick, John; Launay, Helene; de Simone, Alfonso; Haussinger, Daniel; Dobson, Christopher; Vendruscolo, Michele; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John

Citation:

Citation: Wang, Xiaolin; Kirkpatrick, John; Launay, Helene; de Simone, Alfonso; Haussinger, Daniel; Dobson, Christopher; Vendruscolo, Michele; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John. "Probing the dynamic stalk region of the ribosome using solution NMR" Sci. Rep. 9, 13528-13528 (2019).
PubMed: 31537834

Assembly members:

Assembly members:
bL12_S89C, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: purified from the natural source Host organism: Escherichia coli Vector: pET21b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bL12_S89C: MSITKDQIIEAVAAMSVMDV VELISAMEEKFGVSAAAAVA VAAGPVEAAEEKTEFDVILK AAGANKVAVIKAVRGATGLG LKEAKDLVECAPAALKEGVS KDDAEALKKALEEAGAEVEV K

Data sets:

assigned_chemical_shifts
- bL12_S89C_shift_list

Data type	Count
13C chemical shifts	353
15N chemical shifts	117
1H chemical shifts	117

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	bL12, 1	1
2	bL12, 2	1

Entities:

Entity 1, bL12, 1 121 residues - Formula weight is not available

1

MET

SER

ILE

THR

LYS

ASP

GLN

ILE

GLU

2

ALA

VAL

ALA

MET

SER

VAL

MET

ASP

VAL

3

VAL

GLU

LEU

ILE

SER

ALA

MET

GLU

LYS

4

PHE

GLY

VAL

SER

ALA

VAL

ALA

5

VAL

ALA

GLY

PRO

VAL

GLU

ALA

GLU

6

GLU

LYS

THR

GLU

PHE

ASP

VAL

ILE

LEU

LYS

7

ALA

GLY

ALA

ASN

LYS

VAL

ALA

VAL

ILE

8

LYS

ALA

VAL

ARG

GLY

ALA

THR

GLY

LEU

GLY

9

LEU

LYS

GLU

ALA

LYS

ASP

LEU

VAL

GLU

CYS

10

ALA

PRO

ALA

LEU

LYS

GLU

GLY

VAL

SER

11

LYS

ASP

ALA

GLU

ALA

LEU

LYS

ALA

12

LEU

GLU

ALA

GLY

ALA

GLU

VAL

GLU

VAL

13

LYS

Samples:

sample_1: bL12 S89C, [U-13C; U-15N], 0.9 mM; HEPES 10 mM; magnisium chloride 12 mM; ammonium chloride 30 mM; beta-mercaptoethanol 2 mM; DSS 0.001%

sample_conditions_1: ionic strength: 42 mM; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR vVersion2.4, release 2, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks