BMRB Entry 27924

Name: scAtg8(K26P,C33V,G116C)
Published: 2019-08-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27924

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

scAtg8(K26P,C33V,G116C)

Deposition date:

2019-05-23

Original release date:

2019-08-06

Authors:

Royappa, Grace; Zheng, Yumei; Yu, Qiu; Schulman, Brenda

Citation:

Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade" Nat. Commun. 10, 3600-3600 (2019).
PubMed: 31399562

Assembly members:

Assembly members:
scAtg8, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
scAtg8: GSMKSTFKSEYPFEKRKAES ERIADRFPNRIPVIVEKAEK SDIPEIDKRKYLVPADLTVG QFVYVIRKRIMLPPEKAIFI FVNDTLPPTAALMSAIYQEH KDKDGFLYVTYSGENTFC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	192
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	scAtg8	1

Entities:

Entity 1, scAtg8 118 residues - Formula weight is not available

1

GLY

SER

MET

LYS

SER

THR

PHE

LYS

SER

GLU

2

TYR

PRO

PHE

GLU

LYS

ARG

LYS

ALA

GLU

SER

3

GLU

ARG

ILE

ALA

ASP

ARG

PHE

PRO

ASN

ARG

4

ILE

PRO

VAL

ILE

VAL

GLU

LYS

ALA

GLU

LYS

5

SER

ASP

ILE

PRO

GLU

ILE

ASP

LYS

ARG

LYS

6

TYR

LEU

VAL

PRO

ALA

ASP

LEU

THR

VAL

GLY

7

GLN

PHE

VAL

TYR

VAL

ILE

ARG

LYS

ARG

ILE

8

MET

LEU

PRO

GLU

LYS

ALA

ILE

PHE

ILE

9

PHE

VAL

ASN

ASP

THR

LEU

PRO

THR

ALA

10

ALA

LEU

MET

SER

ALA

ILE

TYR

GLN

GLU

HIS

11

LYS

ASP

LYS

ASP

GLY

PHE

LEU

TYR

VAL

THR

12

TYR

SER

GLY

GLU

ASN

THR

PHE

CYS

Samples:

sample_1: scAtg8, [U-100% 13C; U-100% 15N], 0.4 mM; sodium chloride 100 mM; MES 20 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks