BMRB Entry 27922

Name: scAtg3(del 1-18, 86-159, 248-278)
Published: 2019-08-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27922

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

scAtg3(del 1-18, 86-159, 248-278)

Deposition date:

2019-05-23

Original release date:

2019-08-06

Authors:

Royappa, Grace; Zheng, Yumei; Yu, Qiu; Schulman, Brenda

Citation:

Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade" Nat. Commun. 10, 3600-3600 (2019).
PubMed: 31399562

Assembly members:

Assembly members:
Atg3, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Atg3: GSKSTFLTTGQITPEEFVQA GDYLAHMFPTWKWNEESSDI SYRDFLPKNKQFLIIRKVPA DKRAEQAVEAKDMAQERYYD LYIAYSTSYRVPKMYIVGFN SNGSPLSPEQMFEDISADYR TKTATIEKLPFYKNSVLSVS IHPCKHANVMKILLDKVDSL RVDQYLIVFLKFITSVTPSI QHDYTMEGW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	233
15N chemical shifts	143
1H chemical shifts	143

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Atg3	1

Entities:

Entity 1, Atg3 189 residues - Formula weight is not available

1

GLY

SER

LYS

SER

THR

PHE

LEU

THR

GLY

2

GLN

ILE

THR

PRO

GLU

PHE

VAL

GLN

ALA

3

GLY

ASP

TYR

LEU

ALA

HIS

MET

PHE

PRO

THR

4

TRP

LYS

TRP

ASN

GLU

SER

ASP

ILE

5

SER

TYR

ARG

ASP

PHE

LEU

PRO

LYS

ASN

LYS

6

GLN

PHE

LEU

ILE

ARG

LYS

VAL

PRO

ALA

7

ASP

LYS

ARG

ALA

GLU

GLN

ALA

VAL

GLU

ALA

8

LYS

ASP

MET

ALA

GLN

GLU

ARG

TYR

ASP

9

LEU

TYR

ILE

ALA

TYR

SER

THR

SER

TYR

ARG

10

VAL

PRO

LYS

MET

TYR

ILE

VAL

GLY

PHE

ASN

11

SER

ASN

GLY

SER

PRO

LEU

SER

PRO

GLU

GLN

12

MET

PHE

GLU

ASP

ILE

SER

ALA

ASP

TYR

ARG

13

THR

LYS

THR

ALA

THR

ILE

GLU

LYS

LEU

PRO

14

PHE

TYR

LYS

ASN

SER

VAL

LEU

SER

VAL

SER

15

ILE

HIS

PRO

CYS

LYS

HIS

ALA

ASN

VAL

MET

16

LYS

ILE

LEU

ASP

LYS

VAL

ASP

SER

LEU

17

ARG

VAL

ASP

GLN

TYR

LEU

ILE

VAL

PHE

LEU

18

LYS

PHE

ILE

THR

SER

VAL

THR

PRO

SER

ILE

19

GLN

HIS

ASP

TYR

THR

MET

GLU

GLY

TRP

Samples:

sample_1: Atg3, [U-100% 13C; U-100% 15N], 0.75 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - data analysis

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks