BMRB Entry 27916

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27916

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Title:
p50 heterodimer
Deposition date:
2019-05-16
Original release date:
2019-11-08
Authors:
Kohl, Bastian; Granitzka, Vanessa; Singh, Amrinder; Quintas, Pedro; Xiromeriti, Elli; Mortel, Fabian; Wright, Peter; Kroon, Gerard; Dyson, Jane; Stoll, Raphael
Citation:

Citation: Kohl, Bastian; Granitzka, Vanessa; Singh, Amrinder; Quintas, Pedro; Xiromeriti, Elli; Mortel, Fabian; Wright, Peter; Kroon, Gerard; Dyson, Jane; Stoll, Raphael. "Comparison of backbone dynamics of the p50 dimerization domain of NFkB in the homodimeric transcription factor NFkB1 and in its heterodimeric complex with RelA (p65)"  Protein Sci. 28, 2064-2072 (2019).
PubMed: 31587407

Assembly members:

Assembly members:
p50_heterodimer, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-45b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p50_heterodimer: VGTGSNDDDDKAPNASNLKI VRMDRTAGCVTGGEEIYLLC DKVQKDDIQIRFYEEEENGG VWEGFGDFSPTDVHRQFAIV FKTPKYKDVNITKPASVFVQ LRRKSDLETSEPKPFLYYPE I

Data sets:
Data typeCount
13C chemical shifts212
15N chemical shifts108
1H chemical shifts108
T1 relaxation values87
T2 relaxation values87
heteronuclear NOE values87

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1p50 heterodimer, 11
2p50 heterodimer, 21

Entities:

Entity 1, p50 heterodimer, 1 121 residues - Formula weight is not available

p50 dimerization domain in complex with p65 DD

1   VALGLYTHRGLYSERASNASPASPASPASP
2   LYSALAPROASNALASERASNLEULYSILE
3   VALARGMETASPARGTHRALAGLYCYSVAL
4   THRGLYGLYGLUGLUILETYRLEULEUCYS
5   ASPLYSVALGLNLYSASPASPILEGLNILE
6   ARGPHETYRGLUGLUGLUGLUASNGLYGLY
7   VALTRPGLUGLYPHEGLYASPPHESERPRO
8   THRASPVALHISARGGLNPHEALAILEVAL
9   PHELYSTHRPROLYSTYRLYSASPVALASN
10   ILETHRLYSPROALASERVALPHEVALGLN
11   LEUARGARGLYSSERASPLEUGLUTHRSER
12   GLUPROLYSPROPHELEUTYRTYRPROGLU
13   ILE

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks