BMRB Entry 27914

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27914

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for AIMP2 121-320 double-mutant (C205S,C291S)
Deposition date:
2019-05-16
Original release date:
2019-09-20
Authors:
Cho, Hye Young; Jeon, Young Ho; Mushtaq, Ameeq
Citation:

Citation: Lim, Semi; Cho, Hye Young; Kim, Dae Gyu; Roh, Younah; Son, Se-Young; Mushtaq, Ameeq Ul; Kim, Minkyoung; Bhattarai, Deepak; Sivaraman, Aneesh; Lee, Youngjin; Lee, Jihye; Yang, Won Suk; Kim, Hoi Kyoung; Kim, Myung Hee; Lee, Kyeong; Jeon, Young Ho; Kim, Sunghoon. "Targeting the interaction of AIMP2-DX2 with HSP70 suppresses cancer development"  Nat. Chem. Biol. 16, 31-41 (2020).
PubMed: 31792442

Assembly members:

Assembly members:
AIMP2 121-320 double-mutant (C205S,C291S), polymer, 200 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AIMP2 121-320 double-mutant (C205S,C291S): DIVINANPASPPLSLLVLHR LLCEHFRVLSTVHTHSSVKS VPENLLKCFGEQNKKQPRQD YQLGFTLIWKNVPKTQMKFS IQTMSPIEGEGNIARFLFSL FGQKHNAVNATLIDSWVDIA IFQLKEGSSKEKAAVFRSMN SALGKSPWLAGNELTVADVV LWSVLQQIGGSSVTVPANVQ RWMRSCENLAPFNTALKLLK

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts190
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AIMP2 121-320 double-mutant (C205S,C291S)1

Entities:

Entity 1, AIMP2 121-320 double-mutant (C205S,C291S) 200 residues - Formula weight is not available

1   ASPILEVALILEASNALAASNPROALASER
2   PROPROLEUSERLEULEUVALLEUHISARG
3   LEULEUCYSGLUHISPHEARGVALLEUSER
4   THRVALHISTHRHISSERSERVALLYSSER
5   VALPROGLUASNLEULEULYSCYSPHEGLY
6   GLUGLNASNLYSLYSGLNPROARGGLNASP
7   TYRGLNLEUGLYPHETHRLEUILETRPLYS
8   ASNVALPROLYSTHRGLNMETLYSPHESER
9   ILEGLNTHRMETSERPROILEGLUGLYGLU
10   GLYASNILEALAARGPHELEUPHESERLEU
11   PHEGLYGLNLYSHISASNALAVALASNALA
12   THRLEUILEASPSERTRPVALASPILEALA
13   ILEPHEGLNLEULYSGLUGLYSERSERLYS
14   GLULYSALAALAVALPHEARGSERMETASN
15   SERALALEUGLYLYSSERPROTRPLEUALA
16   GLYASNGLULEUTHRVALALAASPVALVAL
17   LEUTRPSERVALLEUGLNGLNILEGLYGLY
18   SERSERVALTHRVALPROALAASNVALGLN
19   ARGTRPMETARGSERCYSGLUASNLEUALA
20   PROPHEASNTHRALALEULYSLEULEULYS

Samples:

sample_1: AIMP2 121-320 double-mutant (C205S,C291S), [U-99% 13C; U-99% 15N], 0.2 mM; Bis-Tris 20 mM; NaCl 50 mM; Glycine 300 mM; mercaptoethanol 7 mM; EDTA 0.1 mM; PMSF 1 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks