BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27913

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal Fragment of a Kinesin KIF4A Variant   PubMed: 32195664

Deposition date: 2019-05-15 Original release date: 2020-03-17

Authors: Wang, Xinru; Peti, Wolfgang; Page, Rebecca

Citation: Wang, Xinru; Garvanska, Dimitriya; Nasa, Isha; Ueki, Yumi; Zhang, Gang; Kettenbach, Arminja; Peti, Wolfgang; Nilsson, Jakob; Page, Rebecca. "A dynamic charge:charge interaction modulates PP2A:B56 interactions"  Elife 9, .-. (2020).

Assembly members:
KIF4A, polymer, 44 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHMT

Entity Sequences (FASTA):
KIF4A: GHMELKHVATEYQENKAPGK KKKRALASNTSFFSGLEPIE EEPE

Data sets:
Data typeCount
13C chemical shifts76
15N chemical shifts39
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIF4A, A1
2KIF4A, B1

Entities:

Entity 1, KIF4A, A 44 residues - Formula weight is not available

Residues 1-3 are from a cloning artifact.

1   GLYHISMETGLULEULYSHISVALALATHR
2   GLUTYRGLNGLUASNLYSALAPROGLYLYS
3   LYSLYSLYSARGALALEUALASERASNTHR
4   SERPHEPHESERGLYLEUGLUPROILEGLU
5   GLUGLUPROGLU

Samples:

sample_1: KIF4A, [U-99% 15N], 0.1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; H2O 90%; D2O 10%

sample_2: KIF4A, [U-99% 13C; U-99% 15N], 3 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Neo 600 MHz

Related Database Links:

UNP O95239

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts