BMRB Entry 27913

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal Fragment of a Kinesin KIF4A Variant
Published: 2020-03-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27913

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal Fragment of a Kinesin KIF4A Variant

Deposition date:

2019-05-15

Original release date:

2020-03-17

Authors:

Wang, Xinru; Peti, Wolfgang; Page, Rebecca

Citation:

Citation: Wang, Xinru; Garvanska, Dimitriya; Nasa, Isha; Ueki, Yumi; Zhang, Gang; Kettenbach, Arminja; Peti, Wolfgang; Nilsson, Jakob; Page, Rebecca. "A dynamic charge:charge interaction modulates PP2A:B56 interactions" Elife 9, .-. (2020).
PubMed: 32195664

Assembly members:

Assembly members:
KIF4A, polymer, 44 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KIF4A: GHMELKHVATEYQENKAPGK KKKRALASNTSFFSGLEPIE EEPE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	76
15N chemical shifts	39
1H chemical shifts	39

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KIF4A, A	1
2	KIF4A, B	1

Entities:

Entity 1, KIF4A, A 44 residues - Formula weight is not available

Residues 1-3 are from a cloning artifact.

1

GLY

HIS

MET

GLU

LEU

LYS

HIS

VAL

ALA

THR

2

GLU

TYR

GLN

GLU

ASN

LYS

ALA

PRO

GLY

LYS

3

LYS

ARG

ALA

LEU

ALA

SER

ASN

THR

4

SER

PHE

SER

GLY

LEU

GLU

PRO

ILE

GLU

5

GLU

PRO

GLU

Samples:

sample_1: KIF4A, [U-99% 15N], 0.1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; H2O 90%; D2O 10%

sample_2: KIF4A, [U-99% 13C; U-99% 15N], 3 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Neo 600 MHz

UNP	O95239
AlphaFold	Q9UMW3