BMRB Entry 27904

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27904

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Fis1 residues 1-125
Deposition date:
2019-05-13
Original release date:
2019-06-13
Authors:
Egner, John; Peterson, Francis
Citation:

Citation: Egner, John; Jensen, Davin; Olp, Michael; Kennedy, Nolan; Volkman, Brian; Peterson, Francis; Smith, Brian; Hill, R. "Development and Validation of 2D Difference Intensity Analysis for Chemical Library Screening by Protein- Detected NMR Spectroscopy"  ChemBioChem 19, 448-458 (2018).
PubMed: 29239081

Assembly members:

Assembly members:
hFis1, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hFis1: MEAVLNELVSVEDLLKFEKK FQSEKAAGSVSKSTQFEYAW CLVRSKYNDDIRKGIVLLEE LLPKGSKEEQRDYVFYLAVG NYRLKEYEKALKYVRGLLQT EPQNNQAKELERLIDKAMKK DGLVG

Data sets:
Data typeCount
13C chemical shifts465
15N chemical shifts118
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hFis11

Entities:

Entity 1, hFis1 125 residues - Formula weight is not available

1   METGLUALAVALLEUASNGLULEUVALSER
2   VALGLUASPLEULEULYSPHEGLULYSLYS
3   PHEGLNSERGLULYSALAALAGLYSERVAL
4   SERLYSSERTHRGLNPHEGLUTYRALATRP
5   CYSLEUVALARGSERLYSTYRASNASPASP
6   ILEARGLYSGLYILEVALLEULEUGLUGLU
7   LEULEUPROLYSGLYSERLYSGLUGLUGLN
8   ARGASPTYRVALPHETYRLEUALAVALGLY
9   ASNTYRARGLEULYSGLUTYRGLULYSALA
10   LEULYSTYRVALARGGLYLEULEUGLNTHR
11   GLUPROGLNASNASNGLNALALYSGLULEU
12   GLUARGLEUILEASPLYSALAMETLYSLYS
13   ASPGLYLEUVALGLY

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks