BMRB Entry 27886

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Shaker-VSD
Published: 2019-05-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27886

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Shaker-VSD

Deposition date:

2019-04-30

Original release date:

2019-05-30

Authors:

Chen, Hongbo; Chanda, Baron; Henzler-Wildman, Katherine

Citation:

Citation: Chen, Hongbo; Pan, Junkun; Gandhi, Disha; Dockendorff, Christopher; Cui, Qiang; Chanda, Baron; Henzler-Wildman, Katherine. "NMR Structural Analysis of Isolated Shaker Voltage-Sensing Domain in LPPG Micelles" Biophys. J. 117, 388-398 (2019).
PubMed: 31301804

Assembly members:

Assembly members:
Shaker-VSD, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Shaker-VSD: GGGGFEYPESSQAARVVAII SVFVILLSIVIFCLETLPEF KHYKVFNTTTNGTKIEEDEV PDITDPFFLIETLCIIWFTF ELTVRFLACPNKLNFCRDVM NVIDIIAIIPYFITLATVVA EEEDTLNLPKAPVSPQDKSS NQAMSLAILRVIRLVRVFRI FKLSRHSKGLQILGRTLKAS MRELG

Data sets:

assigned_chemical_shifts
- WT_Shaker-VSD_1
- WT_Shaker-VSD_2

Data type	Count
13C chemical shifts	524
15N chemical shifts	163
1H chemical shifts	178

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Shaker-VSD	1

Entities:

Entity 1, Shaker-VSD 185 residues - Formula weight is not available

G213, G214, G215, G216 (first four) are resides left after TEV protease cleavage and do not belong to Shaker-VSD.

1

GLY

PHE

GLU

TYR

PRO

GLU

SER

2

SER

GLN

ALA

ARG

VAL

ALA

ILE

3

SER

VAL

PHE

VAL

ILE

LEU

SER

ILE

VAL

4

ILE

PHE

CYS

LEU

GLU

THR

LEU

PRO

GLU

PHE

5

LYS

HIS

TYR

LYS

VAL

PHE

ASN

THR

6

ASN

GLY

THR

LYS

ILE

GLU

ASP

GLU

VAL

7

PRO

ASP

ILE

THR

ASP

PRO

PHE

LEU

ILE

8

GLU

THR

LEU

CYS

ILE

TRP

PHE

THR

PHE

9

GLU

LEU

THR

VAL

ARG

PHE

LEU

ALA

CYS

PRO

10

ASN

LYS

LEU

ASN

PHE

CYS

ARG

ASP

VAL

MET

11

ASN

VAL

ILE

ASP

ILE

ALA

ILE

PRO

12

TYR

PHE

ILE

THR

LEU

ALA

THR

VAL

ALA

13

GLU

ASP

THR

LEU

ASN

LEU

PRO

LYS

14

ALA

PRO

VAL

SER

PRO

GLN

ASP

LYS

SER

15

ASN

GLN

ALA

MET

SER

LEU

ALA

ILE

LEU

ARG

16

VAL

ILE

ARG

LEU

VAL

ARG

VAL

PHE

ARG

ILE

17

PHE

LYS

LEU

SER

ARG

HIS

SER

LYS

GLY

LEU

18

GLN

ILE

LEU

GLY

ARG

THR

LEU

LYS

ALA

SER

19

MET

ARG

GLU

LEU

GLY

Samples:

sample_1: Shaker-VSD, [U-13C; U-15N; U-2H], 0.3 mM; H2O 90%; D2O, [U-2H], 10%; sodium azide 0.05%; TCEP 4 mM; potassium chloride 50 mM; MOPS 100 mM; LPPG ~40 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis, Vranken, Boucher, Stevens, Fogh, Pajon, Llinas, Ulrich, Markley, Ionides, Laue - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks