BMRB Entry 27881

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27881

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Title:
Iron is a physiological ligand of SecA-like Metal Binding Domains
Deposition date:
2019-04-19
Original release date:
2020-03-30
Authors:
Huber, Damon
Citation:

Citation: Cranford-Smith, Tamar; Jamshad, Mohammed; Jeeves, Mark; Chandler, Rachael; Yule, Jack; Robinson, Ashley; Alam, Farhana; Dunne, Karl; Aponte Angarita, Edwin; Alanazi, Mashael; Carter, Cailean; Henderson, Ian; Lovett, Janet; Winn, Peter; Knowles, Timothy; Huber, Damon. "Iron is a ligand of SecA-like metal-binding domains in vivo"  J. Biol. Chem. 295, 7516-7528 (2020).
PubMed: 32241912

Assembly members:

Assembly members:
YecA, polymer, 221 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCA528

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YecA: MKTGPLNESELEWLDDILTK YNTDHAILDVAELDGLLTAV LSSPQEIEPEQWLVAVWGGA DYVPRWASEKEMTRFMNLAF QHMADTAERLNEFPEQFEPL FGLREVDGSELTIVEEWCFG YMRGVALSDWSTLPDSLKPA LEAIALHGTEENFERVEKMS PEAFEESVDAIRLAALDLHA YWMAHPQEKAVQQPIKAEEK PGRNDPCPCGSGKKFKQCCL H

Data sets:
Data typeCount
13C chemical shifts54
15N chemical shifts18
1H chemical shifts18

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1YecA1

Entities:

Entity 1, YecA 221 residues - Formula weight is not available

1   METLYSTHRGLYPROLEUASNGLUSERGLU
2   LEUGLUTRPLEUASPASPILELEUTHRLYS
3   TYRASNTHRASPHISALAILELEUASPVAL
4   ALAGLULEUASPGLYLEULEUTHRALAVAL
5   LEUSERSERPROGLNGLUILEGLUPROGLU
6   GLNTRPLEUVALALAVALTRPGLYGLYALA
7   ASPTYRVALPROARGTRPALASERGLULYS
8   GLUMETTHRARGPHEMETASNLEUALAPHE
9   GLNHISMETALAASPTHRALAGLUARGLEU
10   ASNGLUPHEPROGLUGLNPHEGLUPROLEU
11   PHEGLYLEUARGGLUVALASPGLYSERGLU
12   LEUTHRILEVALGLUGLUTRPCYSPHEGLY
13   TYRMETARGGLYVALALALEUSERASPTRP
14   SERTHRLEUPROASPSERLEULYSPROALA
15   LEUGLUALAILEALALEUHISGLYTHRGLU
16   GLUASNPHEGLUARGVALGLULYSMETSER
17   PROGLUALAPHEGLUGLUSERVALASPALA
18   ILEARGLEUALAALALEUASPLEUHISALA
19   TYRTRPMETALAHISPROGLNGLULYSALA
20   VALGLNGLNPROILELYSALAGLUGLULYS
21   PROGLYARGASNASPPROCYSPROCYSGLY
22   SERGLYLYSLYSPHELYSGLNCYSCYSLEU
23   HIS

Samples:

sample_1: YecA, [U-100% 13C; U-100% 15N], 0.5 mM; MES 20 mM; sodium chloride 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks