BMRB Entry 27870

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for I-domain of Human Integrin alpha M (CD11b)
Published: 2024-01-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27870

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for I-domain of Human Integrin alpha M (CD11b)

Deposition date:

2019-04-04

Original release date:

2024-01-09

Authors:

Feng, Wei; Wang, Xu

Citation:

Citation: Feng, Wei; Nguyen, Hoa; Shen, Di; Deng, Hanqing; Jiang, Zhoumai; Podolnikova, Nataly; Ugarova, Tatiana; Wang, Xu. "Structural Characterization of the Interaction between the alphaMI-Domain of the Integrin Mac-1 (alphaMbeta2) and the Cytokine Pleiotrophin" Biochemistry 60, 182-193 (2021).
PubMed: 33427449

Assembly members:

Assembly members:
aMID, polymer, 194 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHUE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
aMID: EDSDIAFLIDGSGSIIPHDF RRMKEFVSTVMEQLKKSKTL FSLMQYSEEFRIHFTFKEFQ NNPNPRSLVKPITQLLGRTH TATGIRKVVRELFNITNGAR KNAFKILVVITDGEKFGDPL GYEDVIPEADREGVIRYVIG VGDAFRSEKSRQELNTIASK PPRDHVFQVNNFEALKTIQN QLREKIFAIEGTQT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	480
15N chemical shifts	165
1H chemical shifts	165

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	aMID	1

Entities:

Entity 1, aMID 194 residues - Formula weight is not available

Numbering starts at 138.

1

GLU

ASP

SER

ASP

ILE

ALA

PHE

LEU

ILE

ASP

2

GLY

SER

GLY

SER

ILE

PRO

HIS

ASP

PHE

3

ARG

MET

LYS

GLU

PHE

VAL

SER

THR

VAL

4

MET

GLU

GLN

LEU

LYS

SER

LYS

THR

LEU

5

PHE

SER

LEU

MET

GLN

TYR

SER

GLU

PHE

6

ARG

ILE

HIS

PHE

THR

PHE

LYS

GLU

PHE

GLN

7

ASN

PRO

ASN

PRO

ARG

SER

LEU

VAL

LYS

8

PRO

ILE

THR

GLN

LEU

GLY

ARG

THR

HIS

9

THR

ALA

THR

GLY

ILE

ARG

LYS

VAL

ARG

10

GLU

LEU

PHE

ASN

ILE

THR

ASN

GLY

ALA

ARG

11

LYS

ASN

ALA

PHE

LYS

ILE

LEU

VAL

ILE

12

THR

ASP

GLY

GLU

LYS

PHE

GLY

ASP

PRO

LEU

13

GLY

TYR

GLU

ASP

VAL

ILE

PRO

GLU

ALA

ASP

14

ARG

GLU

GLY

VAL

ILE

ARG

TYR

VAL

ILE

GLY

15

VAL

GLY

ASP

ALA

PHE

ARG

SER

GLU

LYS

SER

16

ARG

GLN

GLU

LEU

ASN

THR

ILE

ALA

SER

LYS

17

PRO

ARG

ASP

HIS

VAL

PHE

GLN

VAL

ASN

18

ASN

PHE

GLU

ALA

LEU

LYS

THR

ILE

GLN

ASN

19

GLN

LEU

ARG

GLU

LYS

ILE

PHE

ALA

ILE

GLU

20

GLY

THR

GLN

THR

Samples:

sample_1: aMID, [U-100% 13C; U-100% 15N], 0.4 ± 0.05 mM; HEPES 20 ± 1 mM; sodium chloride 100 ± 5 mM; D2O 10 ± 1 v/v

sample_conditions_1: ionic strength: 0.12 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker Avance 600 MHz