BMRB Entry 27864

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27864

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Title:
New insights into the mechanism and specificity of Pro-Pro endopeptidase-1
Deposition date:
2019-04-01
Original release date:
2019-05-30
Authors:
Diaz, Dolores; Jutten, Linda; Pichlo, Christian; Wojtalla, Fabian; Schachtel, Magdalena; Baumann, Ulrich
Citation:

Citation: Pichlo, Christian; Jutten, Linda; Wojtalla, Fabian; Schachtel, Magdalena; Diaz, Dolores; Baumann, Ulrich. "Molecular determinants of the mechanism and substrate specificity of Clostridium difficile proline-proline endopeptidase-1"  J. Biol. Chem. 294, 11525-11535 (2019).
PubMed: 31182482

Assembly members:

Assembly members:
AP_pep, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AP_pep: EVNAPVP

Data sets:
Data typeCount
1H chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AP_pep1

Entities:

Entity 1, AP_pep 7 residues - Formula weight is not available

1   GLUVALASNALAPROVALPRO

Samples:

sample_1: AP_pep 2 mM; PBS buffer 50 mM

sample_conditions_1: ionic strength: 0.175 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz