BMRB Entry 27860

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27860

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Title:
Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin
Deposition date:
2019-03-30
Original release date:
2019-11-25
Authors:
Charoenwattanasatien, Ratana; Zinzius, Karen; Scholz, Martin; Wicke, Susann; Tanaka, Hideaki; Brandenburg, Johann; Ikegami, Takahisa; Matsumoto, Takashi; Oda, Takashi; Sato, Mamoru; Hippler, Michael; Kurisu, Genji
Citation:

Citation: Charoenwattanasatien, Ratana; Zinzius, Karen; Scholz, Martin; Wicke, Susann; Tanaka, Hideaki; Brandenburg, Johann; Marchetti, Giulia; Ikegami, Takahisa; Matsumoto, Takashi; Oda, Takashi; Sato, Mamoru; Hippler, Michael; Kurisu, Genji. "Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga"  J. Biol. Chem. 295, 170-180 (2020).
PubMed: 31776187

Assembly members:

Assembly members:
calredoxin, polymer, 356 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: green algae   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calredoxin: MCSARSVRVVPRAATFEGLM DDASKAKMEELERRFKMADV DGNGHIDREELRNLLESMES GEVYMMSQHWLPEDELERCM EQYDVNKDGVISFEEFKQII YDGLLLEGTLAEYESAFKAV DKSGNGTIGATELSKLFASL GNPVSLEKLVDLMQMYDKDD SGQIEFPEFLLMFRNSLLDL KDMTTYMTLDEAGAGSSGSL VDAVEGDMTLIFSEEELDAL ISANPDKLVVVFGALTWCRP CKGMQRPVQKLAEHYKDHIV FVKLFGNANKQTKRIFKERF QIRSTPCFITLRKGEPVYTQ TGSNKEKLEAGLRSLIANPP VGMIYPSAEALAALQDPNSS SVDKLAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts15
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1calredoxin1

Entities:

Entity 1, calredoxin 356 residues - Formula weight is not available

1   METCYSSERALAARGSERVALARGVALVAL
2   PROARGALAALATHRPHEGLUGLYLEUMET
3   ASPASPALASERLYSALALYSMETGLUGLU
4   LEUGLUARGARGPHELYSMETALAASPVAL
5   ASPGLYASNGLYHISILEASPARGGLUGLU
6   LEUARGASNLEULEUGLUSERMETGLUSER
7   GLYGLUVALTYRMETMETSERGLNHISTRP
8   LEUPROGLUASPGLULEUGLUARGCYSMET
9   GLUGLNTYRASPVALASNLYSASPGLYVAL
10   ILESERPHEGLUGLUPHELYSGLNILEILE
11   TYRASPGLYLEULEULEUGLUGLYTHRLEU
12   ALAGLUTYRGLUSERALAPHELYSALAVAL
13   ASPLYSSERGLYASNGLYTHRILEGLYALA
14   THRGLULEUSERLYSLEUPHEALASERLEU
15   GLYASNPROVALSERLEUGLULYSLEUVAL
16   ASPLEUMETGLNMETTYRASPLYSASPASP
17   SERGLYGLNILEGLUPHEPROGLUPHELEU
18   LEUMETPHEARGASNSERLEULEUASPLEU
19   LYSASPMETTHRTHRTYRMETTHRLEUASP
20   GLUALAGLYALAGLYSERSERGLYSERLEU
21   VALASPALAVALGLUGLYASPMETTHRLEU
22   ILEPHESERGLUGLUGLULEUASPALALEU
23   ILESERALAASNPROASPLYSLEUVALVAL
24   VALPHEGLYALALEUTHRTRPCYSARGPRO
25   CYSLYSGLYMETGLNARGPROVALGLNLYS
26   LEUALAGLUHISTYRLYSASPHISILEVAL
27   PHEVALLYSLEUPHEGLYASNALAASNLYS
28   GLNTHRLYSARGILEPHELYSGLUARGPHE
29   GLNILEARGSERTHRPROCYSPHEILETHR
30   LEUARGLYSGLYGLUPROVALTYRTHRGLN
31   THRGLYSERASNLYSGLULYSLEUGLUALA
32   GLYLEUARGSERLEUILEALAASNPROPRO
33   VALGLYMETILETYRPROSERALAGLUALA
34   LEUALAALALEUGLNASPPROASNSERSER
35   SERVALASPLYSLEUALAALAALALEUGLU
36   HISHISHISHISHISHIS

Samples:

sample_1: calredoxin, [13C]-Met, 0.3 ± 0.03 mM; HEPES 20 ± 2 mM; sodium chloride 150 ± 15 mM; DTT 0.5 ± 0.05 mM; calcium chloride 2.5 ± 0.25 mM; EGTA 10 ± 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker Avance 950 MHz