BMRB Entry 27856
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27856
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Wang, Zhihao; Zhao, Siyu; Jiang, Songzi; Wang, Yawen; Buck, Martin; Matthews, Steve; Liu, Bing. "Resonance assignments of N-terminal receiver domain of sigma factor S regulator RssB from Escherichia coli." Biomol. NMR Assign. 13, 333-337 (2019).
PubMed: 31228091
Assembly members:
RssB_N-terminal_1-125, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-46
Entity Sequences (FASTA):
RssB_N-terminal_1-125: MTQPLVGKQILIVEDEQVFR
SLLDSWFSSLGATTVLAADG
VDALELLGGFTPDLMICDIA
MPRMNGLKLLEHIRNRGDQT
PVLVISATENMADIAKALRL
GVEDVLLKPVKDLNRLREMV
FACLY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 507 |
| 15N chemical shifts | 126 |
| 1H chemical shifts | 897 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RssB | 1 |
Entities:
Entity 1, RssB 125 residues - Formula weight is not available
| 1 | MET | THR | GLN | PRO | LEU | VAL | GLY | LYS | GLN | ILE | ||||
| 2 | LEU | ILE | VAL | GLU | ASP | GLU | GLN | VAL | PHE | ARG | ||||
| 3 | SER | LEU | LEU | ASP | SER | TRP | PHE | SER | SER | LEU | ||||
| 4 | GLY | ALA | THR | THR | VAL | LEU | ALA | ALA | ASP | GLY | ||||
| 5 | VAL | ASP | ALA | LEU | GLU | LEU | LEU | GLY | GLY | PHE | ||||
| 6 | THR | PRO | ASP | LEU | MET | ILE | CYS | ASP | ILE | ALA | ||||
| 7 | MET | PRO | ARG | MET | ASN | GLY | LEU | LYS | LEU | LEU | ||||
| 8 | GLU | HIS | ILE | ARG | ASN | ARG | GLY | ASP | GLN | THR | ||||
| 9 | PRO | VAL | LEU | VAL | ILE | SER | ALA | THR | GLU | ASN | ||||
| 10 | MET | ALA | ASP | ILE | ALA | LYS | ALA | LEU | ARG | LEU | ||||
| 11 | GLY | VAL | GLU | ASP | VAL | LEU | LEU | LYS | PRO | VAL | ||||
| 12 | LYS | ASP | LEU | ASN | ARG | LEU | ARG | GLU | MET | VAL | ||||
| 13 | PHE | ALA | CYS | LEU | TYR |
Samples:
sample_1: RssB N-terminal 1-125, [U-100% 13C; U-100% 15N], 0.5 mM; sodium chloride 250 mM; TRIS 50 mM; TCEP 1 mM
sample_2: RssB N-terminal 1-125, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; sodium chloride 250 mM; TRIS 50 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 250 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks