BMRB Entry 27851

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27851

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Title:
Backbone resonance assignments of the PRY-SPRY domain of RNF135
Deposition date:
2019-03-23
Original release date:
2019-05-22
Authors:
Zhang, Danting; Wei, Huan; Kuang, Zhihe
Citation:

Citation: Zhang, Danting; Wei, Huan; Xue, Hongjuan; Guo, Shujun; Wu, Bin; Kuang, Zhihe. "Backbone 1H, 13C, and 15N resonance assignments of the PRY-SPRY domain of RNF135"  Biomol. NMR Assignments 13, 299-304 (2019).
PubMed: 31065957

Assembly members:

Assembly members:
PRY-SPRY_domain_of_RNF135, polymer, 209 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PRY-SPRY_domain_of_RNF135: MGSSSSCPLPDQSHPALRRA SRFAQWAIHPTFNLKSLSCS LEVSKDSRTVTVSHRPQPYR WSCERFSTSQVLCSQALSSG KHYWEVDTRNCSHWAVGVAS WEMSRDQVLGRTMDSCCVEW KGTSQLSAWHMVKETVLGSD RPGVVGIWLNLEEGKLAFYS VDNQEKLLYECTISASSPLY PAFWLYGLHPGNYLIIKQVK VGSHHHHHH

Data sets:
Data typeCount
13C chemical shifts543
15N chemical shifts174
1H chemical shifts350

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNF135 PRY-SPRY monomer1

Entities:

Entity 1, RNF135 PRY-SPRY monomer 209 residues - Formula weight is not available

1   METGLYSERSERSERSERCYSPROLEUPRO
2   ASPGLNSERHISPROALALEUARGARGALA
3   SERARGPHEALAGLNTRPALAILEHISPRO
4   THRPHEASNLEULYSSERLEUSERCYSSER
5   LEUGLUVALSERLYSASPSERARGTHRVAL
6   THRVALSERHISARGPROGLNPROTYRARG
7   TRPSERCYSGLUARGPHESERTHRSERGLN
8   VALLEUCYSSERGLNALALEUSERSERGLY
9   LYSHISTYRTRPGLUVALASPTHRARGASN
10   CYSSERHISTRPALAVALGLYVALALASER
11   TRPGLUMETSERARGASPGLNVALLEUGLY
12   ARGTHRMETASPSERCYSCYSVALGLUTRP
13   LYSGLYTHRSERGLNLEUSERALATRPHIS
14   METVALLYSGLUTHRVALLEUGLYSERASP
15   ARGPROGLYVALVALGLYILETRPLEUASN
16   LEUGLUGLUGLYLYSLEUALAPHETYRSER
17   VALASPASNGLNGLULYSLEULEUTYRGLU
18   CYSTHRILESERALASERSERPROLEUTYR
19   PROALAPHETRPLEUTYRGLYLEUHISPRO
20   GLYASNTYRLEUILEILELYSGLNVALLYS
21   VALGLYSERHISHISHISHISHISHIS

Samples:

sample_1: PRY-SPRY domain of RNF135, [U-99% 13C; U-99% 15N], 0.4 mM; H2O 95%; D2O, [U-99% 2H], 5%; sodium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.02%

sample_2: PRY-SPRY domain of RNF135, [U-99% 13C; U-99% 15N; U-75% 2H], 0.4 mM; H2O 95%; D2O, [U-99% 2H], 5%; sodium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Agilent DD2 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks