BMRB Entry 27848

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments of co-repressor nuclear receptor interacting domain N-CoR NID
Published: 2019-07-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27848

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments of co-repressor nuclear receptor interacting domain N-CoR NID

Deposition date:

2019-03-21

Original release date:

2019-07-25

Authors:

Barthe, Philippe; Sibille, Nathalie; Bernado, Pau

Citation:

Citation: Cordeiro, Tiago; Sibille, Nathalie; Germain, Pierre; Barthe, Philippe; Boulahtouf, Abdelhay; Allemand, Frederic; Bailly, Remy; Vivat, Valerie; Ebel, Christine; Barducci, Alessandro; Bourguet, William; le Maire, Albane; Bernado, Pau. "Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression" Structure 27, 1270-1285 (2019).
PubMed: 31178221

Assembly members:

Assembly members:
N-CoR_NID, polymer, 269 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-CoR_NID: GSQVPRTHRLITLADHICQI ITQDFARNQVPSQPSTSTFQ TSPSALSSTPVRTKTSSRYS PESQSQTVLHPRPGPRVSPE NLVDKSRGSRPGKSPERSHI PSEPYEPISPPQGPAVHEKQ DSMLLLSQRGVDPAEQRSDS RSPGSISYLPSFFTKLESTS PMVKSKKQEIFRKLNSSGGG DSDMAAAQPGTEIFNLPAVT TSGAVSSRSHSFADPASNLG LEDIIRKALMGSFDDKVEDH GVVMSHPVGIMPGSASTSVV TSSEARRDE

Data sets:

assigned_chemical_shifts
- N-CoR_NID_assigned_CS_list

Data type	Count
13C chemical shifts	617
15N chemical shifts	183
1H chemical shifts	183

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-CoR NID	1

Entities:

Entity 1, N-CoR NID 269 residues - Formula weight is not available

Nuclear receptor corepressor 1 (N-CoR) Nuclear Receptor Interacting Domain (NID).

1

GLY

SER

GLN

VAL

PRO

ARG

THR

HIS

ARG

LEU

2

ILE

THR

LEU

ALA

ASP

HIS

ILE

CYS

GLN

ILE

3

ILE

THR

GLN

ASP

PHE

ALA

ARG

ASN

GLN

VAL

4

PRO

SER

GLN

PRO

SER

THR

SER

THR

PHE

GLN

5

THR

SER

PRO

SER

ALA

LEU

SER

THR

PRO

6

VAL

ARG

THR

LYS

THR

SER

ARG

TYR

SER

7

PRO

GLU

SER

GLN

SER

GLN

THR

VAL

LEU

HIS

8

PRO

ARG

PRO

GLY

PRO

ARG

VAL

SER

PRO

GLU

9

ASN

LEU

VAL

ASP

LYS

SER

ARG

GLY

SER

ARG

10

PRO

GLY

LYS

SER

PRO

GLU

ARG

SER

HIS

ILE

11

PRO

SER

GLU

PRO

TYR

GLU

PRO

ILE

SER

PRO

12

PRO

GLN

GLY

PRO

ALA

VAL

HIS

GLU

LYS

GLN

13

ASP

SER

MET

LEU

SER

GLN

ARG

GLY

14

VAL

ASP

PRO

ALA

GLU

GLN

ARG

SER

ASP

SER

15

ARG

SER

PRO

GLY

SER

ILE

SER

TYR

LEU

PRO

16

SER

PHE

THR

LYS

LEU

GLU

SER

THR

SER

17

PRO

MET

VAL

LYS

SER

LYS

GLN

GLU

ILE

18

PHE

ARG

LYS

LEU

ASN

SER

GLY

19

ASP

SER

ASP

MET

ALA

GLN

PRO

GLY

20

THR

GLU

ILE

PHE

ASN

LEU

PRO

ALA

VAL

THR

21

THR

SER

GLY

ALA

VAL

SER

ARG

SER

HIS

22

SER

PHE

ALA

ASP

PRO

ALA

SER

ASN

LEU

GLY

23

LEU

GLU

ASP

ILE

ARG

LYS

ALA

LEU

MET

24

GLY

SER

PHE

ASP

LYS

VAL

GLU

ASP

HIS

25

GLY

VAL

MET

SER

HIS

PRO

VAL

GLY

ILE

26

MET

PRO

GLY

SER

ALA

SER

THR

SER

VAL

27

THR

SER

GLU

ALA

ARG

ASP

GLU

Samples:

sample_1: N-CoR NID, [U-100% 13C; U-100% 15N], 600 uM; TRIS Bis 50 mM; NaCl 150 mM; EDTA 2 mM; TCEP 2 mM

N-CoR_NID_buffer: ionic strength: 150 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HNCO	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HN(CA)CO	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HNCA	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HN(CO)CA	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HNCACB	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HN(CO)CACB	sample_1	isotropic	N-CoR_NID_buffer
3D BT-iHNCA	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HN(COCA)NH	sample_1	isotropic	N-CoR_NID_buffer
3D BT-HN(CA)NH	sample_1	isotropic	N-CoR_NID_buffer

Software:

TOPSPIN, Bruker Biospin - collection

GIFA, Delsuc - chemical shift calculation, processing

CINDY, Andre PADILLA (CBS) - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks