BMRB Entry 27839

Name: Iga1 Protease G5 domain
Published: 2019-04-24

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PDB ID: 6oh1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27839
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Iga1 Protease G5 domain

Deposition date:

2019-03-19

Original release date:

2019-04-24

Authors:

Eisenmesser, Elan; Chi, Ying-Chih Chi; Paukovich, Natasia; Redzic, Jasmina; Rahkola, Jeremy; Janoff, Edward

Citation:

Citation: Paukovich, Natasia; Redzic, Jasmina; Chi, Ying-Chih; Rahkola, Jeremy; Issaian, Aaron; Blue, Ashley; Hansen, Kirk; Janoff, Edward; Eisenmesser, Elan. "Streptococcus pneumoniae G5 domains bind different ligands" Protein Sci. 28, 1797-1805 (2019).
PubMed: 31390088

Assembly members:

Assembly members:
IgA1P-G5, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pj401

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IgA1P-G5: GSHNKPELLYREETIETKID FQEEIQENPDLAEGTVRVKQ EGKLGKKVEIVRIFSVNKEE VSREIVSTSTTAPSPRIVEK GTKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	329
15N chemical shifts	78
1H chemical shifts	461

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IgA1 protease G5 monomer	1

Entities:

Entity 1, IgA1 protease G5 monomer 84 residues - Formula weight is not available

1

GLY

SER

HIS

ASN

LYS

PRO

GLU

LEU

TYR

2

ARG

GLU

THR

ILE

GLU

THR

LYS

ILE

ASP

3

PHE

GLN

GLU

ILE

GLN

GLU

ASN

PRO

ASP

4

LEU

ALA

GLU

GLY

THR

VAL

ARG

VAL

LYS

GLN

5

GLU

GLY

LYS

LEU

GLY

LYS

VAL

GLU

ILE

6

VAL

ARG

ILE

PHE

SER

VAL

ASN

LYS

GLU

7

VAL

SER

ARG

GLU

ILE

VAL

SER

THR

SER

THR

8

THR

ALA

PRO

SER

PRO

ARG

ILE

VAL

GLU

LYS

9

GLY

THR

LYS

Samples:

sample_1: IgA1P-G5, [U-13C; U-15N; U-2H], 0.5 mM; MES 50 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 25 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian DRX 900 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks