BMRB Entry 27836

Name: Backbone 1H, 15N, and 13C Chemical Shifts of Myosin VI Medial Tail Domain
Published: 2019-06-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27836

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N, and 13C Chemical Shifts of Myosin VI Medial Tail Domain

Deposition date:

2019-03-14

Original release date:

2019-06-26

Authors:

Barnes, C. Ashley; Shen, Yang; Ying, Jinfa; Takagi, Yasuharu; Torchia, Dennis; Sellers, James; Bax, Ad

Citation:

Citation: Barnes, C. Ashley; Shen, Yang; Ying, Jinfa; Takagi, Yasuharu; Torchia, Dennis; Sellers, James; Bax, Ad. "Remarkable Rigidity of the Single alpha-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy." J. Am. Chem. Soc. 141, 9004-9017 (2019).
PubMed: 31117653

Assembly members:

Assembly members:
MT_Domain, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MT_Domain: KQQEEEAERLRRIQEEMEKE RKRREEDEQRRRKEEEERRM KLEMEAKRKQEEEERKKRED DEKRIQAE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	118
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MT Domain	1

Entities:

Entity 1, MT Domain 68 residues - Formula weight is not available

1

LYS

GLN

GLU

ALA

GLU

ARG

LEU

2

ARG

ILE

GLN

GLU

MET

GLU

LYS

GLU

3

ARG

LYS

ARG

GLU

ASP

GLU

GLN

ARG

4

ARG

LYS

GLU

ARG

MET

5

LYS

LEU

GLU

MET

GLU

ALA

LYS

ARG

LYS

GLN

6

GLU

ARG

LYS

ARG

GLU

ASP

7

ASP

GLU

LYS

ARG

ILE

GLN

ALA

GLU

Samples:

sample_1: sodium phosphate 20 mM; EDTA 2 mM; MT Domain, [U-13C; U-15N; U-2H], 1 mM

sample_2: sodium phosphate 20 mM; EDTA 2 mM; MT Domain, [U-100% 2H; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.3; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
4D HN-HN NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking, processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks