BMRB Entry 27834

Name: Sequence Specific Assignment of OSR1 C-terminal Domain.
Published: 2019-05-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27834

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence Specific Assignment of OSR1 C-terminal Domain.

Deposition date:

2019-03-12

Original release date:

2019-05-22

Authors:

AlAmri, Mubarak; Jeeves, Mark; Mehellou, Youcef

Citation:

Citation: AlAmri, Mubarak; Jeeves, Mark; Mehellou, Youcef. "Sequence Specific Assignment and Determination of OSR1 C-terminal Domain Structure by NMR" Biochem. Biophys. Res. Commun. 512, 338-343 (2019).
PubMed: 30894278

Assembly members:

Assembly members:
OSR1, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OSR1: PISLVLRLRNSKKELNDIRF EFTPGRDTAEGVSQELISAG LVDGRDLVIVAANLQKIVEE PQSNRSVTFKLASGVEGSDI PDDGKLIGFAQLSIS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	247
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminal Domain	1

Entities:

Entity 1, C-terminal Domain 95 residues - Formula weight is not available

1

PRO

ILE

SER

LEU

VAL

LEU

ARG

LEU

ARG

ASN

2

SER

LYS

GLU

LEU

ASN

ASP

ILE

ARG

PHE

3

GLU

PHE

THR

PRO

GLY

ARG

ASP

THR

ALA

GLU

4

GLY

VAL

SER

GLN

GLU

LEU

ILE

SER

ALA

GLY

5

LEU

VAL

ASP

GLY

ARG

ASP

LEU

VAL

ILE

VAL

6

ALA

ASN

LEU

GLN

LYS

ILE

VAL

GLU

7

PRO

GLN

SER

ASN

ARG

SER

VAL

THR

PHE

LYS

8

LEU

ALA

SER

GLY

VAL

GLU

GLY

SER

ASP

ILE

9

PRO

ASP

GLY

LYS

LEU

ILE

GLY

PHE

ALA

10

GLN

LEU

SER

ILE

SER

Samples:

sample_1: OSR1, [U-100% 13C; U-100% 15N], 0.2 mM; TRIS, [U-2H], 50 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

CcpNmr_Analysis, ranken, Boucher, Stevens, Fogh, Pajon, Llinas, Ulrich, Markley, Ionides, Laue - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks