BMRB Entry 27832

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27832

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Title:
Methyl Chemical shift Assignment of EIC from the thermophile thermoanaerobacter tengcongenesis
Deposition date:
2019-03-07
Original release date:
2019-05-22
Authors:
Dotas, Rochelle; Venditti, Vincenzo
Citation:

Citation: Dotas, Rochelle; Venditti, Vincenzo. "Resonance assignment of the 128 kDa enzyme I dimer from Thermoanaerobacter tengcongensis"  Biomol. NMR Assignments 13, 287-293 (2019).
PubMed: 31025174

Assembly members:

Assembly members:
tEIC_methyls, polymer, 314 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Caldanaerobacter subterraneus   Taxonomy ID: 911092   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermoanaerobacter tengcongenesis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tEIC_methyls: MAETPDGKKVMLAANIGTPK DVASALANGAEGVGLFRTEF LYMDRNSLPSEEEQFEAYKE VVEKMGGRPVTIRTLDIIGD KELPYLDMPKEMNPFLGYRA IRLCLDRPDIFKTQLRAILR ASAYGNVQIMYPMISSVEEV RKANSILEEVKAELDREGVK YDKEIKVGIMVEIPSAAVTA DILAKEVDFFSIGTNDLTQY TLAVDRMNEHVKEYYQPFHP AILRLVKMVIDAAHKEGKFA AMCGEMAGDPLAAVILLGLG LDEFSMSATSIPEIKNIIRN VEYEKAKEIAEKALNMSEAR EIEKMMKDVIKDIG

Data sets:
Data typeCount
13C chemical shifts94
1H chemical shifts282

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1tEIC methyls1

Entities:

Entity 1, tEIC methyls 314 residues - Formula weight is not available

1   METALAGLUTHRPROASPGLYLYSLYSVAL
2   METLEUALAALAASNILEGLYTHRPROLYS
3   ASPVALALASERALALEUALAASNGLYALA
4   GLUGLYVALGLYLEUPHEARGTHRGLUPHE
5   LEUTYRMETASPARGASNSERLEUPROSER
6   GLUGLUGLUGLNPHEGLUALATYRLYSGLU
7   VALVALGLULYSMETGLYGLYARGPROVAL
8   THRILEARGTHRLEUASPILEILEGLYASP
9   LYSGLULEUPROTYRLEUASPMETPROLYS
10   GLUMETASNPROPHELEUGLYTYRARGALA
11   ILEARGLEUCYSLEUASPARGPROASPILE
12   PHELYSTHRGLNLEUARGALAILELEUARG
13   ALASERALATYRGLYASNVALGLNILEMET
14   TYRPROMETILESERSERVALGLUGLUVAL
15   ARGLYSALAASNSERILELEUGLUGLUVAL
16   LYSALAGLULEUASPARGGLUGLYVALLYS
17   TYRASPLYSGLUILELYSVALGLYILEMET
18   VALGLUILEPROSERALAALAVALTHRALA
19   ASPILELEUALALYSGLUVALASPPHEPHE
20   SERILEGLYTHRASNASPLEUTHRGLNTYR
21   THRLEUALAVALASPARGMETASNGLUHIS
22   VALLYSGLUTYRTYRGLNPROPHEHISPRO
23   ALAILELEUARGLEUVALLYSMETVALILE
24   ASPALAALAHISLYSGLUGLYLYSPHEALA
25   ALAMETCYSGLYGLUMETALAGLYASPPRO
26   LEUALAALAVALILELEULEUGLYLEUGLY
27   LEUASPGLUPHESERMETSERALATHRSER
28   ILEPROGLUILELYSASNILEILEARGASN
29   VALGLUTYRGLULYSALALYSGLUILEALA
30   GLULYSALALEUASNMETSERGLUALAARG
31   GLUILEGLULYSMETMETLYSASPVALILE
32   LYSASPILEGLY

Samples:

sample_1: tEIC methyls, [U-2H; U-13C, methyl], 1 mM; DTT 2 mM; TRIS 20 mM; EDTA 1 mM; MgCl2 4 mM; NaCl 100 mM; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz