BMRB Entry 27820

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27820

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of ITSN1 L_SH3D20
Deposition date:
2019-03-04
Original release date:
2019-03-15
Authors:
Gerth, Fabian; Jaepel, Maria; Sticht, Jana; Schmitt, Xiao; Kuropka, Benno; Driller, Jan; Loll, Bernhard; Wahl, Markus; Pagel, Kevin; Haucke, Volker; Freund, Christian
Citation:

Citation: Gerth, Fabian; Jaepel, Maria; Sticht, Jana; Schmitt, Xiao; Kuropka, Benno; Driller, Jan; Loll, Bernhard; Wahl, Markus; Pagel, Kevin; Haucke, Volker; Freund, Christian. "Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain."  Structure 27, 977-987 (2019).
PubMed: 31031201

Assembly members:

Assembly members:
ITSN1_L-SH3AD20, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ITSN1_L-SH3AD20: GSHMQAPWSTAEKGPLTISA QENVKVVYYRALYPFESRSH DEITIQPGDIVMVDESQTGE PGWLGGELKGKTGWFPANYA EKIPE

Data sets:
Data typeCount
13C chemical shifts76
15N chemical shifts71
1H chemical shifts71

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1L-SH3AD201

Entities:

Entity 1, L-SH3AD20 85 residues - Formula weight is not available

This is the short splice isoform of the SH3A domain (SH3AD20) with N-terminal extension, residues GSHM at the N-terminus are non-native.

1   GLYSERHISMETGLNALAPROTRPSERTHR
2   ALAGLULYSGLYPROLEUTHRILESERALA
3   GLNGLUASNVALLYSVALVALTYRTYRARG
4   ALALEUTYRPROPHEGLUSERARGSERHIS
5   ASPGLUILETHRILEGLNPROGLYASPILE
6   VALMETVALASPGLUSERGLNTHRGLYGLU
7   PROGLYTRPLEUGLYGLYGLULEULYSGLY
8   LYSTHRGLYTRPPHEPROALAASNTYRALA
9   GLULYSILEPROGLU

Related Database Links:

UNP Q15811-3
AlphaFold Q9UQ92

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks