BMRB Entry 27817
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27817
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gerth, Fabian; Jaepel, Maria; Sticht, Jana; Schmitt, Xiao; Kuropka, Benno; Driller, Jan; Loll, Bernhard; Wahl, Markus; Pagel, Kevin; Haucke, Volker; Freund, Christian. "Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain." Structure 27, 977-987 (2019).
PubMed: 31031201
Assembly members:
ITSN1_SH3AD20, polymer, 65 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
ITSN1_SH3AD20: GSHMKVVYYRALYPFESRSH
DEITIQPGDIVMVDESQTGE
PGWLGGELKGKTGWFPANYA
EKIPE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 61 |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 57 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3AD20 | 1 |
Entities:
Entity 1, SH3AD20 65 residues - Formula weight is not available
This is the short splice isoform of the SH3A domain (SH3AD20), residues GSHM at the N-terminus are non-native.
| 1 | GLY | SER | HIS | MET | LYS | VAL | VAL | TYR | TYR | ARG | ||||
| 2 | ALA | LEU | TYR | PRO | PHE | GLU | SER | ARG | SER | HIS | ||||
| 3 | ASP | GLU | ILE | THR | ILE | GLN | PRO | GLY | ASP | ILE | ||||
| 4 | VAL | MET | VAL | ASP | GLU | SER | GLN | THR | GLY | GLU | ||||
| 5 | PRO | GLY | TRP | LEU | GLY | GLY | GLU | LEU | LYS | GLY | ||||
| 6 | LYS | THR | GLY | TRP | PHE | PRO | ALA | ASN | TYR | ALA | ||||
| 7 | GLU | LYS | ILE | PRO | GLU |
Samples:
sample_1: ITSN1 SH3AD20, [U-100% 13C; U-100% 15N], 400 uM; NaCl 50 mM
all_samples_conditions: pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | all_samples_conditions |
| 3D HNCA | sample_1 | isotropic | all_samples_conditions |
| 3D HN(CO)CA | sample_1 | isotropic | all_samples_conditions |
Software:
CcpNMR_Analysis, CCPN - chemical shift assignment
TOPSPIN v3, Bruker - collection, processing, refinement
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks