BMRB Entry 27816
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27816
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Schneider, Tim; Hung, Lee-Hsueh; Aziz, Masood; Wilmen, Anna; Thaum, Stephanie; Wagner, Jacqueline; Janowski, Robert; Mueller, Simon; Schreiner, Silke; Friedhoff, Peter; Huettelmaier, Stefan; Niessing, Dierk; Sattler, Michael; Schlundt, Andreas; Bindereif, Albrecht. "Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3." Nat. Commun. 10, 2266-2266 (2019).
PubMed: 31118463
Assembly members:
IMP3_(IGF2BP3)_KH1-2_delta2, polymer, 168 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETTrx1a
Entity Sequences (FASTA):
IMP3_(IGF2BP3)_KH1-2_delta2: GAMAKPCDLPLRLLVPTQFV
GAIIGKEGATIRNITKQTQS
KIDVHRKENAGAAEKSITIL
STPEGTSAACKSILEIMHKE
AQDIKFTEEIPLKILAHNNF
VGRLIGDDGRNLKKIEQDTD
TKITISPLQELTLYNPERTI
TVKGNVETCAKAEEEIMKKI
RESYENDI
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 156 |
| 1H chemical shifts | 156 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IMP3 KH1-2 delta2 | 1 |
Entities:
Entity 1, IMP3 KH1-2 delta2 168 residues - Formula weight is not available
carries K294D and E295D double mutation as compared to wt
| 1 | GLY | ALA | MET | ALA | LYS | PRO | CYS | ASP | LEU | PRO | ||||
| 2 | LEU | ARG | LEU | LEU | VAL | PRO | THR | GLN | PHE | VAL | ||||
| 3 | GLY | ALA | ILE | ILE | GLY | LYS | GLU | GLY | ALA | THR | ||||
| 4 | ILE | ARG | ASN | ILE | THR | LYS | GLN | THR | GLN | SER | ||||
| 5 | LYS | ILE | ASP | VAL | HIS | ARG | LYS | GLU | ASN | ALA | ||||
| 6 | GLY | ALA | ALA | GLU | LYS | SER | ILE | THR | ILE | LEU | ||||
| 7 | SER | THR | PRO | GLU | GLY | THR | SER | ALA | ALA | CYS | ||||
| 8 | LYS | SER | ILE | LEU | GLU | ILE | MET | HIS | LYS | GLU | ||||
| 9 | ALA | GLN | ASP | ILE | LYS | PHE | THR | GLU | GLU | ILE | ||||
| 10 | PRO | LEU | LYS | ILE | LEU | ALA | HIS | ASN | ASN | PHE | ||||
| 11 | VAL | GLY | ARG | LEU | ILE | GLY | ASP | ASP | GLY | ARG | ||||
| 12 | ASN | LEU | LYS | LYS | ILE | GLU | GLN | ASP | THR | ASP | ||||
| 13 | THR | LYS | ILE | THR | ILE | SER | PRO | LEU | GLN | GLU | ||||
| 14 | LEU | THR | LEU | TYR | ASN | PRO | GLU | ARG | THR | ILE | ||||
| 15 | THR | VAL | LYS | GLY | ASN | VAL | GLU | THR | CYS | ALA | ||||
| 16 | LYS | ALA | GLU | GLU | GLU | ILE | MET | LYS | LYS | ILE | ||||
| 17 | ARG | GLU | SER | TYR | GLU | ASN | ASP | ILE |
Samples:
sample_1: IMP3 (IGF2BP3) KH1-2 delta2, [U-99% 13C; U-99% 15N], 0.5 mM; BisTris 20 mM; sodium chloride 150 mM; TCEP 2 mM; sodium azide 0.02%
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker - collection, processing
CCPNMR_Analysis, CCPN - chemical shift assignment, chemical shift calculation, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
- Bruker Avance 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks