BMRB Entry 27785

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27785

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Title:
1H, 13C and 15N resonance assignments for the first and second CUE domains from chromatin remodeler SMARCAD1
Deposition date:
2019-02-13
Original release date:
2019-04-12
Authors:
Redfield, Christina; Biasutto, Antonio; Mancini, Erika; West, Phillip
Citation:

Citation: Biasutto, Antonio; West, Phillip; Mancini, Erika; Redfield, Christina. "1H, 13C and 15N resonance assignments for the tandem CUE domains from chromatin remodeler SMARCAD1"  Biomol. NMR Assignments 13, 261-265 (2019).
PubMed: 30919308

Assembly members:

Assembly members:
dCUE, polymer, 166 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-DUET-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dCUE: MGSSHHHHHHSQDPDISELE DLSELEDLKDAKLQTLKELF PQRSDNDLLKLIESTSTMDG AIAAALLMFGDAGGGPRKRK LSSSSEPYEEDEFNDDQSIK KTRLDHGEESNESAESSNNW EKQESIVLKLQKEFPNFDKQ ELREVLKEHEWMYTEALESL KVFAED

Data sets:
Data typeCount
13C chemical shifts550
15N chemical shifts139
1H chemical shifts831

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dCUE1

Entities:

Entity 1, dCUE 166 residues - Formula weight is not available

Residues 130-143 are a non-native N-terminal His6 tag and a thrombin cleavage site.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERGLNASPPROASPILESERGLULEUGLU
3   ASPLEUSERGLULEUGLUASPLEULYSASP
4   ALALYSLEUGLNTHRLEULYSGLULEUPHE
5   PROGLNARGSERASPASNASPLEULEULYS
6   LEUILEGLUSERTHRSERTHRMETASPGLY
7   ALAILEALAALAALALEULEUMETPHEGLY
8   ASPALAGLYGLYGLYPROARGLYSARGLYS
9   LEUSERSERSERSERGLUPROTYRGLUGLU
10   ASPGLUPHEASNASPASPGLNSERILELYS
11   LYSTHRARGLEUASPHISGLYGLUGLUSER
12   ASNGLUSERALAGLUSERSERASNASNTRP
13   GLULYSGLNGLUSERILEVALLEULYSLEU
14   GLNLYSGLUPHEPROASNPHEASPLYSGLN
15   GLULEUARGGLUVALLEULYSGLUHISGLU
16   TRPMETTYRTHRGLUALALEUGLUSERLEU
17   LYSVALPHEALAGLUASP

Samples:

sample_1: dCUE, [U-99% 15N], 0.5 ± 0.05 mM; sodium phosphate 20 mM; sodium chloride 100 mM

sample_2: dCUE, [U-99% 13C; U-99% 15N], 0.5 ± 0.05 mM; sodium phosphate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 160 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D CBCANHsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Home-built OMEGA 750 MHz

Related Database Links:

UNP Q9H4L7
AlphaFold Q9ULU7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks