BMRB Entry 27784

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27784

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Title:
Backbone 1H, 15N, 13C Chemical Shift Assignment of the Pfr state of monomeric PAS-GAF-PHY from Deinococcus radiodurans
Deposition date:
2019-02-13
Original release date:
2019-12-10
Authors:
Gustavsson, Emil; Isaksson, Linnea; Persson, Cecilia; Mayzel, Maxim; Brath, Ulrika; Vrhovac, Lidija; Ihalainen, Janne; Karlsson, Goran; Orekhov, Vladislav; Westenhoff, Sebastian
Citation:

Citation: Gustavsson, Emil; Isaksson, Linnea; Persson, Cecilia; Mayzel, Maxim; Brath, Ulrika; Vrhovac, Lidija; Ihalainen, Janne; Karlsson, Goran; Orekhov, Vladislav; Westenhoff, Sebastian. "A photoactivated soft-to-rigid transition in the bacteriophytochrome PHY tongue - a solution NMR study of a 57 kDa protein"  Biophys. J. ., .-..

Assembly members:

Assembly members:
Phytochrome, polymer, 509 residues, 57000 Da.

Natural source:

Natural source:   Common Name: Deinococcus radiodurans   Taxonomy ID: 1299   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Deinococcus radiodurans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Phytochrome: MSRDPLPFFPPLYLGGPEIT TENCEREPIHIPGSIQPHGA LLTADGHSGEVLQMSLNAAT FLGQEPTVLRGQTLAALLPE QWPALQAALPPGCPDALQYR ATLDWPAAGHLSLTVHRVGE LLILEFEPTEAWDSTGPHAL RNAMSALESAPNLRALAEVA TQTVRELTGFDRVMLYKFAP DATGEVIAEARREGLHAFLG HRFPASDIPAQARALYTRHL LRLTADTRAAAVPLDPVLNP QTNAPTPLGGAVLRATSPMH MQYLRNMGVGSSLSVSVVVG GQLWGLIACHHQTPYVLPPD LRTTLEYLGRELSEQVQVKE AADVAAFRQSLREHHARVAL AAAHSLSPHDTLSDPALDLL GLMRAGGLILRFEGRWQTLG EVPPAPAVDALLAWLETQPG ALVQTDALGQLWPAGADLAP SAAGLLAISVGEGWSECLVW LRPELRLEVAWGGATPDQAK DDLGPRHSFDTYLEEKRGYA EPWHPGEIEEAQDLRDTLTG ALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts701
15N chemical shifts243
1H chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAS-GAF-PHY1

Entities:

Entity 1, PAS-GAF-PHY 509 residues - 57000 Da.

1   METSERARGASPPROLEUPROPHEPHEPRO
2   PROLEUTYRLEUGLYGLYPROGLUILETHR
3   THRGLUASNCYSGLUARGGLUPROILEHIS
4   ILEPROGLYSERILEGLNPROHISGLYALA
5   LEULEUTHRALAASPGLYHISSERGLYGLU
6   VALLEUGLNMETSERLEUASNALAALATHR
7   PHELEUGLYGLNGLUPROTHRVALLEUARG
8   GLYGLNTHRLEUALAALALEULEUPROGLU
9   GLNTRPPROALALEUGLNALAALALEUPRO
10   PROGLYCYSPROASPALALEUGLNTYRARG
11   ALATHRLEUASPTRPPROALAALAGLYHIS
12   LEUSERLEUTHRVALHISARGVALGLYGLU
13   LEULEUILELEUGLUPHEGLUPROTHRGLU
14   ALATRPASPSERTHRGLYPROHISALALEU
15   ARGASNALAMETSERALALEUGLUSERALA
16   PROASNLEUARGALALEUALAGLUVALALA
17   THRGLNTHRVALARGGLULEUTHRGLYPHE
18   ASPARGVALMETLEUTYRLYSPHEALAPRO
19   ASPALATHRGLYGLUVALILEALAGLUALA
20   ARGARGGLUGLYLEUHISALAPHELEUGLY
21   HISARGPHEPROALASERASPILEPROALA
22   GLNALAARGALALEUTYRTHRARGHISLEU
23   LEUARGLEUTHRALAASPTHRARGALAALA
24   ALAVALPROLEUASPPROVALLEUASNPRO
25   GLNTHRASNALAPROTHRPROLEUGLYGLY
26   ALAVALLEUARGALATHRSERPROMETHIS
27   METGLNTYRLEUARGASNMETGLYVALGLY
28   SERSERLEUSERVALSERVALVALVALGLY
29   GLYGLNLEUTRPGLYLEUILEALACYSHIS
30   HISGLNTHRPROTYRVALLEUPROPROASP
31   LEUARGTHRTHRLEUGLUTYRLEUGLYARG
32   GLULEUSERGLUGLNVALGLNVALLYSGLU
33   ALAALAASPVALALAALAPHEARGGLNSER
34   LEUARGGLUHISHISALAARGVALALALEU
35   ALAALAALAHISSERLEUSERPROHISASP
36   THRLEUSERASPPROALALEUASPLEULEU
37   GLYLEUMETARGALAGLYGLYLEUILELEU
38   ARGPHEGLUGLYARGTRPGLNTHRLEUGLY
39   GLUVALPROPROALAPROALAVALASPALA
40   LEULEUALATRPLEUGLUTHRGLNPROGLY
41   ALALEUVALGLNTHRASPALALEUGLYGLN
42   LEUTRPPROALAGLYALAASPLEUALAPRO
43   SERALAALAGLYLEULEUALAILESERVAL
44   GLYGLUGLYTRPSERGLUCYSLEUVALTRP
45   LEUARGPROGLULEUARGLEUGLUVALALA
46   TRPGLYGLYALATHRPROASPGLNALALYS
47   ASPASPLEUGLYPROARGHISSERPHEASP
48   THRTYRLEUGLUGLULYSARGGLYTYRALA
49   GLUPROTRPHISPROGLYGLUILEGLUGLU
50   ALAGLNASPLEUARGASPTHRLEUTHRGLY
51   ALALEUGLUHISHISHISHISHISHIS

Samples:

Pfr_state: Phytochrome, [U-13C; U-15N; U-2H], 0.8 mM; sodium chloride 50 mM; sodium phosphate 25 mM; azide 0.02%; Complete EDTA free 2 tablet/100mL

sample_conditions_1: ionic strength: 0.025 M; pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOPfr_stateisotropicsample_conditions_1
3D HNCAPfr_stateisotropicsample_conditions_1
3D HNCACBPfr_stateisotropicsample_conditions_1
3D HN(CO)CAPfr_stateisotropicsample_conditions_1
3D HN(CO)CACBPfr_stateisotropicsample_conditions_1
3D HN(CA)COPfr_stateisotropicsample_conditions_1
2D 1H-15N TROSYPfr_stateisotropicsample_conditions_1
3D 1H-15N NOESY TROSYPfr_stateisotropicsample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks