BMRB Entry 27776

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27776

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Title:
ssNMR assignment of membrane embedded Rhomboid protease GlpG
Deposition date:
2019-02-05
Original release date:
2020-02-28
Authors:
Shi, Chaowei; Chevelkov, Veniamin; Bohg, Claudia; Lange, Sascha; Lange, Adam
Citation:

Citation: Shi, Chaowei; Oster, Carl; Bohg, Claudia; Li, Longmei; Lange, Sascha; Chevelkov, Veniamin; Lange, Adam. "Structure and Dynamics of the Rhomboid Protease GlpG in Liposomes Studied by Solid-State NMR"  J. Am. Chem. Soc. 141, 17314-17321 (2019).
PubMed: 31603315

Assembly members:

Assembly members:
GlpG, polymer, 211 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GlpG: MGSSHHHHHHSSGLVPRGSH MAALRERAGPVTWVMMIACV VVFIAMQILGDQEVMLWLAW PFDPTLKFEFWRYFTHALMH FSLMHILFNLLWWWYLGGAV EKRLGSGKLIVITLISALLS GYVQQKFSGPWFGGLSGVVY ALMGYVWLRGERDPQSGIYL QRGLIIFALIWIVAGWFDLF GMSMANGAHIAGLAVGLAMA FVDSLNARKRK

Data sets:
Data typeCount
13C chemical shifts279
15N chemical shifts90
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GlpG1

Entities:

Entity 1, GlpG 211 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALAALALEUARGGLUARGALAGLYPRO
4   VALTHRTRPVALMETMETILEALACYSVAL
5   VALVALPHEILEALAMETGLNILELEUGLY
6   ASPGLNGLUVALMETLEUTRPLEUALATRP
7   PROPHEASPPROTHRLEULYSPHEGLUPHE
8   TRPARGTYRPHETHRHISALALEUMETHIS
9   PHESERLEUMETHISILELEUPHEASNLEU
10   LEUTRPTRPTRPTYRLEUGLYGLYALAVAL
11   GLULYSARGLEUGLYSERGLYLYSLEUILE
12   VALILETHRLEUILESERALALEULEUSER
13   GLYTYRVALGLNGLNLYSPHESERGLYPRO
14   TRPPHEGLYGLYLEUSERGLYVALVALTYR
15   ALALEUMETGLYTYRVALTRPLEUARGGLY
16   GLUARGASPPROGLNSERGLYILETYRLEU
17   GLNARGGLYLEUILEILEPHEALALEUILE
18   TRPILEVALALAGLYTRPPHEASPLEUPHE
19   GLYMETSERMETALAASNGLYALAHISILE
20   ALAGLYLEUALAVALGLYLEUALAMETALA
21   PHEVALASPSERLEUASNALAARGLYSARG
22   LYS

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