BMRB Entry 27775

Name: HLS3_Olduvai
Published: 2019-07-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27775

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HLS3_Olduvai

Deposition date:

2019-02-04

Original release date:

2019-07-02

Authors:

Henen, Morkos; Schmitt, Lauren; Issaian, Aaron; Vogeli, Beat; Hansen, Kirk

Citation:

Citation: Issaian, Aaron; Schmitt, Lauren; Born, Alexandra; Nichols, Parker; Sikela, James; Hansen, Kirk; Vogeli, Beat; Henen, Morkos. "Solution NMR backbone assignment reveals interaction-free tumbling of human lineage-specific Olduvai protein domains" Biomol. NMR Assign. 13, 339-343 (2019).
PubMed: 31264103

Assembly members:

Assembly members:
HLS3, polymer, 94 residues, 10553.3948 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HLS3: SAAAASSASLEVDRIKKDQE EEEDQGPPCPRLSRELLEVV EPEVLQDSLDRCYSTPSSCL EQPDSCQPYGSSFYALEEKH VGFSLDLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	188
15N chemical shifts	64
1H chemical shifts	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HLS3	1

Entities:

Entity 1, HLS3 94 residues - 10553.3948 Da.

1

SER

ALA

SER

ALA

SER

LEU

2

GLU

VAL

ASP

ARG

ILE

LYS

ASP

GLN

GLU

3

GLU

ASP

GLN

GLY

PRO

CYS

PRO

4

ARG

LEU

SER

ARG

GLU

LEU

GLU

VAL

5

GLU

PRO

GLU

VAL

LEU

GLN

ASP

SER

LEU

ASP

6

ARG

CYS

TYR

SER

THR

PRO

SER

CYS

LEU

7

GLU

GLN

PRO

ASP

SER

CYS

GLN

PRO

TYR

GLY

8

SER

PHE

TYR

ALA

LEU

GLU

LYS

HIS

9

VAL

GLY

PHE

SER

LEU

ASP

LEU

GLU

HIS

10

HIS

Samples:

sample_1: HLS3, [U-100% 13C; U-100% 15N], 140 uM; sodium chloride 50 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
Expt_3 (H[N[co[{CA\|ca[C]}]]])	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, data analysis, processing

NMR spectrometers:

Varian INOVA 900 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks