BMRB Entry 27772

Name: 1H, 15N,13C assignments of the Solanum tuberosum Plant Specific Insert at pH 2.0
Published: 2020-07-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27772

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N,13C assignments of the Solanum tuberosum Plant Specific Insert at pH 2.0

Deposition date:

2019-02-01

Original release date:

2020-07-09

Authors:

Tian, Jingxin; Yada, Rickey; Wang, Shenlin

Citation:

Citation: Zhao, Xiaoli; Tian, Jenny; Yu, Hua; Bryksa, Brian; Dupuis, John; Ou, Xiuyuan; Qian, Zhaohui; Song, Chen; Wang, Shenlin; Yada, Rickey. "Insights into the mechanism of membrane fusion induced by the plant defense element, Plant Specific Insert" J. Biol. Chem. ., .-. (2020).

Assembly members:

Assembly members:
PSI, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: potato Taxonomy ID: 4113 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Solanum tuberosum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Rosetta-gami

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PSI: GSAMAIVSMECKTIVSQYGE MIWDLLVSGVRPDQVCSQAG LCFVDGAQHVSSNIKTVVER ETEGSSVGEAPLCTACEMAV VWMQNQLKQEGTKEKVLEYV NQLCEKIP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	239
15N chemical shifts	90
1H chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PSI	1

Entities:

Entity 1, PSI 108 residues - Formula weight is not available

1

GLY

SER

ALA

MET

ALA

ILE

VAL

SER

MET

GLU

2

CYS

LYS

THR

ILE

VAL

SER

GLN

TYR

GLY

GLU

3

MET

ILE

TRP

ASP

LEU

VAL

SER

GLY

VAL

4

ARG

PRO

ASP

GLN

VAL

CYS

SER

GLN

ALA

GLY

5

LEU

CYS

PHE

VAL

ASP

GLY

ALA

GLN

HIS

VAL

6

SER

ASN

ILE

LYS

THR

VAL

GLU

ARG

7

GLU

THR

GLU

GLY

SER

VAL

GLY

GLU

ALA

8

PRO

LEU

CYS

THR

ALA

CYS

GLU

MET

ALA

VAL

9

VAL

TRP

MET

GLN

ASN

GLN

LEU

LYS

GLN

GLU

10

GLY

THR

LYS

GLU

LYS

VAL

LEU

GLU

TYR

VAL

11

ASN

GLN

LEU

CYS

GLU

LYS

ILE

PRO

Samples:

sample_1: PSI, [U-100% 13C; U-100% 15N], 0.2 mM

sample_conditions_1: ionic strength: 30 mM; pH: 2.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks