BMRB Entry 27764

Name: FoxM1 Transactivation Domain, Phosphorylated form
Published: 2019-06-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27764

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

FoxM1 Transactivation Domain, Phosphorylated form

Deposition date:

2019-01-22

Original release date:

2019-06-13

Authors:

Marceau, Aimee; Brison, Caileen; Nerli, Santrupti; Arsenault, Heather; McShane, Andrew; Chen, Eefei; Lee, Hsiau-Wei; Benanti, Jennifer; Sgourakis, Nikolaos; Rubin, Seth

Citation:

Citation: Marceau, Aimee; Brison, Caileen; Nerli, Santrupti; Arsenault, Heather; McShane, Andrew; Chen, Eefei; Lee, Hsiau-Wei; Benanti, Jennifer; Sgourakis, Nikolaos; Rubin, Seth. "An order-to-disorder structural switch activates the FoxM1 transcription factor." Elife 8, e46131-e46131 (2019).
PubMed: 31134895

Assembly members:

Assembly members:
Phosphorylated_FoxM1_TAD, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source: Common Name: zebrafish Taxonomy ID: 7955 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Danio rerio

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Phosphorylated_FoxM1_TAD: GEFGAANRXLTEGFVLDTMN DXLSKILVDIXFSGLEDEDL GMGNISWXQFIPELK

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	153
15N chemical shifts	54
1H chemical shifts	55

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Phosphorylated FoxM1 TAD	1

Entities:

Entity 1, Phosphorylated FoxM1 TAD 55 residues - Formula weight is not available

1

GLY

GLU

PHE

GLY

ALA

ASN

ARG

SEP

LEU

2

THR

GLU

GLY

PHE

VAL

LEU

ASP

THR

MET

ASN

3

ASP

SEP

LEU

SER

LYS

ILE

LEU

VAL

ASP

ILE

4

SEP

PHE

SER

GLY

LEU

GLU

ASP

GLU

ASP

LEU

5

GLY

MET

GLY

ASN

ILE

SER

TRP

SEP

GLN

PHE

6

ILE

PRO

GLU

LEU

LYS

Samples:

sample_1: Phosphorylated FoxM1 TAD, [U-100% 13C; U-100% 15N], 450 mM; sodium chloride 100 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks